1. To evaluate and optimalize a 3 Tesla MR protocol for the determination of the lipid composition of abdominal adipose tissue; by means of spectroscopy. 2.Determination of the reproducibility of MR-spectroscopy of abdominal adipose tissue. Both…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
dysfunctie van abdominaal vetweefsel
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
1. Unit defining the outcome of the MR-spectroscopic measurements. Again,
because MR-spectroscopy has not been performed on abdominal adipose tissue
before, no predefined unit is available.
With the spectroscopy measurements of abdominal adipose tissue, we want to be
able to distinguish different lipid components at their known place in the
spectrum. These peaks should include:
- the water peak at 4.65 ppm
- the triglyceride peak at 0.9 and 1.3 ppm
- the total unsaturated fatty acids peak at 5.4 ppm
- the poly unsaturated fatty acids peak at 2.8 ppm
These peaks should be easily determinable and should not overlap each other ,
making calculation of AUC*s possible. If these conditions are met, data will be
regarded as adequate. If other peaks are present in the spectra of the
abdominal adipose tissue, these will be analyzed in the same manner. With
calculated AUC*s of each of these components ratio*s of the lipid components
relative to water can be calculated.
These ratio*s in abdominal adipose tissue can be compared to the same ratio*s
obtained from spectroscopic measurements in other tissues, for example
subcutaneous adipose tissue or hepatic tissue. When related to other tissue*s a
comparison can be made regarding the amount of the different lipidcomponents.
For this study, because we do not know what will be the most appropriate tissue
to compare the spectroscopic measurements of abdominal adipose tissue to, we
want to perform spectroscopy of liver, subcutaneous adipose tissue, skeletal
muscle and spleen.
Because we want to determine whether the ratio's differ when a person has a
small or large amount of abdominal adipose tissue, half of the participantis
will have a waistcircumference of >88cm (women) or >102cm (men).
2. Reproducibility of spectroscopic measurements
Both interscan (i.e. intra-individual) and interobserver reproducibility will
be defined using Bland-Altman plots and interclass correlation coefficients.
Secondary outcome
1. To compare spectroscopic measurements of participants with and without
(laboratory) measurements of metabolic syndrome.
2. To compare adipokine profiles of participants with spectroscopic
measurements (only when technique seems feasible).
Background summary
Dysfunctionality of adipose tissue is currently determined by measuring plasma
levels of adipokines/cytokines obtained in peripheral blood or by sampling
adipose tissue during abdominal surgery. Both methods have considerable
disadvantages; peripheral bloodsamples might not be representative for the
actual metabolic activity of abdominal adipose tissue and adipose tissue
biopsies can only be performed when patients undergo surgery for other reasons.
To be informed about the (dys) functionality of abdominal adipose tissue may
help to understand pathophysiology, may direct therapy against obesity,
diabetes and cardiovascular diseases and may provide tools for predicting the
risk of diabetes and vascular diseases. MR-spectroscopy of the abdomen might be
a non-invase and reproducible way to obtain information on the metabolic
activity of abdominal adipose tissue.
Study objective
1. To evaluate and optimalize a 3 Tesla MR protocol for the determination of
the lipid composition of abdominal adipose tissue; by means of spectroscopy.
2.Determination of the reproducibility of MR-spectroscopy of abdominal adipose
tissue. Both interscan (i.e. intra-individual; comparing the two MR scans of
each participant) and interobserver reproducibility will be determined.
Study design
A cross-sectional study with 2 separate MR-spectroscopy measurements per
individual for purposes of scanoptimalization and reproducibility.
Study burden and risks
Participants will be asked to visit the hospital twice, during the first visit
a MR-spectroscopy will take place and anthropometric measurements will be
taken. Besides those measurements, 13 ml of blood will be drawn during the
first visit; 1 tube will be used for direct measurement of glucose, insulin and
lipidprofile. The rest of the blood will be stored to be able to determine a
adipokineprofile when the technique of spectroscopy seems feasible.
During the second visit, the MR-spectroscopy will be repeated, no other tests
will take place. Participants will be asked to fast overnight before the MRI
scans take place.
Making these MRI scans and performing the other measurements gives a very
small, almost negligible burden for the participant. As far as we know, all of
the procedures used in this study have no short- or longterm side-effects.
Heidelberglaan 100
3584 CX Utrecht
NL
Heidelberglaan 100
3584 CX Utrecht
NL
Listed location countries
Age
Inclusion criteria
1. Between ages of 18 and 65 years.
2. Healthy volunteers
3. Male or female
Exclusion criteria
1. Known cardiovascular disease
2. Known renal, liver or pulmonary disease
3. Use of medication (except for oral anticonceptives, PPI*s, inhalation medication or topical unguents)
4. Pregnancy or lactation
5. Severe claustrophobia
6. Waist circumference too large to fit MRI. (> 200cm)
7. Metallic devices in the body
Design
Recruitment
Medical products/devices used
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL34282.041.10 |