To study the validity and usefulness of three immunological assays to improve knowledge on the course and diagnosis of sesame seed allergy in sesame seed allergic patients with proven anaphylaxis. In the first place, the CAP inhibition assay is used…
ID
Source
Brief title
Condition
- Allergic conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
BAT: Upregulation of CD-63 on basophils, converted to the percentage activated
basophils. CAP: Percentage inhibition, calculated by amount of sIgE bound to
CAPs. Immunoblot: Thickness of protein bands visible on the Immunoblot (kDa).
Secondary outcome
NVT
Background summary
Sesame seed anaphylaxis is potentially life-threatening. Patients need to have
life-long dietary restrictions and emergency medication, which is often a
social burden for these patients. Accurate diagnosis is necessary, to prevent
redundant dietary restrictions. The golden standard for diagnosis of food
allergy is the double-blind, placebo-controlled food challenge, which is
potentially dangerous and expensive. Alternative diagnostic methods are serum
specific Immunoglobulin E (IgE) testing and skin prick tests (SPTs). However,
these tests can give high numbers of false positive results. Another promising
method in the diagnosis of food allergy is the basophil activation test (BAT).
The number of studies investigating this method in relation to sesame seed
allergy in particular is limited. More research is needed to confirm the
usefulness of BAT and to improve the testing procedure. In addition, to study
the course of sesame seed allergy, application of immunoblotting and the
immunological assay CAP inhibition could be useful. Until now, the relatively
new method of CAP inhibition is only tested on fig allergens. Therefore, more
research should be aimed at investigating these assays to improve knowledge on
the course and diagnosis of sesame seed allergy.
Study objective
To study the validity and usefulness of three immunological assays to improve
knowledge on the course and diagnosis of sesame seed allergy in sesame seed
allergic patients with proven anaphylaxis. In the first place, the CAP
inhibition assay is used to study the primary sensitization allergen in these
sesame seed allergic patients. Secondly, the immunological assay BAT is tested
for its reliability in the diagnosis of sesame seed allergy. Finally, the
Immunoblot is used to investigate the sesame seed specific IgE profile of the
patient and the potential relationship with the atopic profile and clinical
history.
Study design
Observational pilot study.
Study burden and risks
Participants will have to donate a small amount of blood once. The burden and
risks associated with participation are negligible.
Philips van Leydenlaan 15
Nijmegen 6525 EX
NL
Philips van Leydenlaan 15
Nijmegen 6525 EX
NL
Listed location countries
Age
Inclusion criteria
known sesame seed allergy (medical history and sIgE detection by Skin test or RAST).
Exclusion criteria
Age below 15.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL42526.091.12 |