To evaluate the possible role of brain lactate on hepatic VLDL-TG secretion in the liver in healthy men.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
vet metabolisme
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
VLDL-TG secretion
Secondary outcome
scd-1, lactate in brain
Background summary
In present studies a more prominent role of the brains has been shown in the
regulation of the lipidmetabolism. Intraventricular and intravenous lactate
infusion showed a strong decrease in liver VLDL-TG secretion in healthy rats.
The possible association between brain lactate and increased VLDL-TG in this
disease is not known.
.
Study objective
To evaluate the possible role of brain lactate on hepatic VLDL-TG secretion in
the liver in healthy men.
Study design
case control intervention study that will consist of three visits
Intervention
lactate infusion, glycerol bolus
Study burden and risks
A total of 490 ml venous blood will be withdrawn. 240 ml per research day and
10 ml at visit 1.
A haematoma can occur at the place of the needle.
Lactate has been extensively used in studies in the same concentration and is
frequently used in the hospital for patients with extracellular depletion. Side
effects of lactate are in the protocol. Plasma lactate will be kept stable at 3
mmol/L by bedside with a 10 minutes-interval.
Labelled glycerol behaves in vivo as its unlabeled isotopomer and therefore has
no side effects
No harmful effects are known from MRS scanning of the brain.
Meibergdreef 9
1105 AZ Amsterdam
NL
Meibergdreef 9
1105 AZ Amsterdam
NL
Listed location countries
Age
Inclusion criteria
Healthy men (BMI<26), 18-60 yrs
Exclusion criteria
Panic disorder, renal and liver dysfunction (creatinine > 150 umol/L, elevated liver enzymes, known or previous cardiovascular diseases, DM II. In case of the MRS: claustrophobia, foreign metal devices
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL22088.018.08 |