To investigate the effects of 5 days of one legged knee immobilization with or without daily NMES on quadriceps CSA, lean mass and muscle fiber characteristics.
ID
Source
Brief title
Condition
- Other condition
- Protein and amino acid metabolism disorders NEC
- Muscle disorders
Synonym
Health condition
muscle metabolism
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Primary: Quadriceps CSA
Secondary outcome
quadriceps lean mass, type I and II muscle fiber CSA and SC content, muscle
strength.
Background summary
Situations such as injury or illness can necessitate a period of muscle disuse
(limb immobilization or bed-rest) in otherwise healthy individuals. Even brief
periods of such muscle disuse lead to a rapid loss of muscle mass and,
consequently, functional strength. Accordingly, feasible strategies for
attenuating the loss of muscle during disuse need to be pursued. Local
neuromuscular electrical stimulation (NMES) offers such a potential strategy
but, as yet, remains untested during muscle disuse in healthy humans.Therefore,
the aim of the present study is to investigate whether twice daily local
(quadriceps) NMES attenuates muscle loss during 5 days of one legged knee
immobilization.
Study objective
To investigate the effects of 5 days of one legged knee immobilization with or
without daily NMES on quadriceps CSA, lean mass and muscle fiber
characteristics.
Study design
Randomized, parallel (two groups) study design.
Intervention
Five days of knee immobilization with or without twice daily NMES.
Study burden and risks
The risks involved in participating in this experiment are minimal. Muscle
biopsies will be taken through a small (5 mm) incision, following local
anesthetics of the skin and muscle fascia, and will heal completely. Muscle
biopsies will only be obtained by an experienced physician. Five days of limb
immobilization via a knee brace will impair subject*s mobility for this period.
However, to minimize any risk of injury subjects will not be allowed to drive a
vehicle or ride a bicycle and will have daily contact with the investigators
experimenters. The expected loss of muscle mass and strength following
immobilization will be rapidly (<2 weeks) regained due to the inclusion of
young, healthy volunteers.
Universiteitssingel 50 - room 2.208
6229 ER
NL
Universiteitssingel 50 - room 2.208
6229 ER
NL
Listed location countries
Age
Inclusion criteria
- Male
- Aged between from 18-35 years
- BMI above 18.5 and below 30 kg/m2
Exclusion criteria
- Any family history of thromboses
- All co-morbidities interacting with mobility and muscle metabolism of the lower limbs (e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis).
- Diagnosed impaired renal or liver function
- Myocardial infarction within the last 3 years
- Use of anti-coagulants
Design
Recruitment
Medical products/devices used
Followed up by the following (possibly more current) registration
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Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL38575.068.11 |