To investigate whether elderly individuals lose muscle mass at a greater rate than young individuals during 5 days of one legged knee immobilization, and whether dietary protein supplementation can alleviate such muscle loss in the elderly.
ID
Source
Brief title
Condition
- Other condition
- Protein and amino acid metabolism disorders NEC
- Muscle disorders
Synonym
Health condition
muscle metabolism
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Quadriceps cross sectional area (CSA).
Secondary outcome
Leg lean mass, type I and II muscle fiber CSA and satellite cell content,
muscle strength, mRNA and protein expression of anabolic signaling proteins.
Background summary
Situations such as injury or illness can necessitate a period of muscle disuse
(limb immobilization or bed-rest) in otherwise healthy individuals. Even brief
periods of such muscle disuse lead to a rapid loss of muscle mass and,
consequently, functional strength. This is of major relevance to elderly
individuals who already have a compromised functional capacity. However, it
remains unknown whether elderly individuals are more susceptible to muscle loss
during disuse compared to the young. Furthermore, whether muscle loss can be
alleviated during disuse by increasing dietary protein intake remains
equivocal.
Study objective
To investigate whether elderly individuals lose muscle mass at a greater rate
than young individuals during 5 days of one legged knee immobilization, and
whether dietary protein supplementation can alleviate such muscle loss in the
elderly.
Study design
Randomized, parallel (three groups) study design.
Intervention
Five days of one-legged knee immobilization in young individuals (YNG group),
and five days of one-legged knee immobilization in elderly individuals with
(OLD-PRO) or without (OLD-CON) twice daily dietary protein supplementation.
Study burden and risks
The risks involved in participating in this experiment are minimal. Muscle
biopsies will be taken through a small (5 mm) incision, following local
anesthetics of the skin and muscle fascia, and will heal completely. Muscle
biopsies will only be obtained by an experienced physician. Five days of limb
immobilization via a full leg cast will impair subject*s mobility for this
period. However, to minimize any risk of injury subjects will not be allowed to
drive a vehicle or ride a bicycle and will have daily contact with the
investigators. The 5 day immobilization period will lead to a loss of muscle
mass and strength. However, the expected loss of muscle mass and strength
following immobilization will be rapidly (<2 weeks) regained due to the
inclusion of only healthy volunteers.
Universiteitssingel 50 - room 2.208
6229 ER
NL
Universiteitssingel 50 - room 2.208
6229 ER
NL
Listed location countries
Age
Inclusion criteria
Male
Aged from 18-35 years and from 65-75 years
18.5 < BMI < 30 kg/m2
Exclusion criteria
Smoking
Performing regular resistance training in the previous 6 months
Hypertension (according to WHO criteria) and/or cardiovascular disease
Any back/leg/knee/shoulder complaints which may interfere with the use of crutches
Systemic use of antibiotics within 3 weeks prior to the study visit
Current systemic use of corticosteroids, growth hormone, testosterone, immunosuppressants or insulin
Type 2 diabetes mellitus
Any family history of thrombosis
All co-morbidities interacting with mobility and muscle metabolism of the lower limbs (e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis)
Myocardial infarction within the last 3 years
Use of anti-coagulants
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL39878.068.12 |