We aim to determine an association between the presence of complement binding anti-paternal HLA-C antibodies and preeclampsia.
ID
Source
Brief title
Condition
- Maternal complications of pregnancy
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Presence of HLA-C IgG1 and/or IgG3
Secondary outcome
Presence of HLA-C IgG and IgM
Presence of HLA class I and II antibodies
Background summary
Preeclampsia is a relatively common but potentially dangerous disorder in human
pregnancy, significantly contributing to maternal and neonatal morbidity and
mortality. Recent evidence suggests preeclampsia to be associated with
classical complement activation, with preference of C4d deposits at the
syncytiotrophoblast at the fetal-maternal interface. The question is whether
these complement deposits result from binding with antibody-antigen immune
complexes at the trophoblasts. Therefore, in this study we focus on the
detection of antibodies that target the paternal human leukocyte antigens (HLA)
on the surface of the trophoblast: HLA-C.
Study objective
We aim to determine an association between the presence of complement binding
anti-paternal HLA-C antibodies and preeclampsia.
Study design
We prospectively collect maternal blood samples and umbilical cord blood of
women after uncomplicated, or preeclampstic pregnancies. We compare the
presence of complement binding HLA-C antibodies in a retrospective case-control
design.
Study burden and risks
The subjects will not be exposed to activities for this research. The maternal
bloodsamples are taken immediately before or after delivery by competent
nursesand during routine bloodsamples. Risks and side effects of taking blood
are considered as nonexistent.
The umbilical cord blood is taken from the umbilical cord after delivery, there
are no risks or side effect expected with this sampling.
Participation in the study will not directly benefit the subject, but the
results may in future be important for other patients
Albinusdreef 2
Leiden 2333 ZA
NL
Albinusdreef 2
Leiden 2333 ZA
NL
Listed location countries
Age
Inclusion criteria
We select primiparous women with an uncomplicated, term pregnancy (control) and preeclampsia (case) who deliver at the Leiden University Medical Center. Preeclampsia is defined as gestational hypertension (systolic pressure >= 140 mmHg and/or diastolic pressure >= 90 mmHg detected after 20th week of gestation) or worsening of preexistent hypertension and proteinuria (>= 0.3 gr/l/24hours).
Exclusion criteria
Maternal autoimmune diseases as antiphospholipid syndrome or SLE, drugs, use of immunosuppressive medication, intrauterine infections and bloodtransfusions or organ transplantions in medical history.
Design
Recruitment
metc-ldd@lumc.nl
metc-ldd@lumc.nl
Followed up by the following (possibly more current) registration
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Other (possibly less up-to-date) registrations in this register
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In other registers
| Register | ID |
|---|---|
| CCMO | NL44413.058.13 |