We aim to test our hypothesis after collecting venous blood samples from adults who were exposed to malaria as expatriate living in malaria-endemic regions. After anonimisation of blood samples, functional immunological assays will be performed in…
ID
Source
Brief title
Condition
- Protozoal infectious disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
A single venous blood sample (32mL) will be taken along with a short
questionnaire with questions about malaria exposure and age at first malaria
exposure. After anonimisation, peripheral blood mononuclear cell (PBMC these
samples will be used to: i) assess functional transmission blocking immunity by
standard membrane feeding assay; ii) determine (a reduction in) hepatocyte
invasion by sporozoites in the presence of isolated immunoglobulins (IgG), iii)
determine the production of interferon-gamma (IFN-*), tumor necrosis factor *
(TNF-*) and interleukin-2 (IL-2) in PBMC subpopulations after stimulation with
plasmodium infected red blood cells, sporozoites and malaria antigens; iv)
determine the antibody profile against a panel of sexual stage, bloodstage and
liverstage antigens by protein microarray.
Secondary outcome
-
Background summary
Immunity to malaria develops slowly. Even after repeated exposure sterile
immunity that prevents infected mosquito bites from establishing blood-stage
infection is rarely observed in endemic populations. Also transmission blocking
immunity whereby human antibodies interfere with parasite development in
mosquitoes is rarely seen in endemic populations. By contrast, sterile immunity
and functional transmission-blocking immunity are commonly observed in adult
populations who are exposed to malaria in experimental infections, during
travelling or after exposure to malaria while living in malaria-endemic regions
as expatriate. We hypothesize that controlled malaria exposure in adulthood,
when the immune system has reached a certain level of maturation, results in a
more efficient immunity to different malaria life stages compared to exposure
from at an earlier age in life.
Study objective
We aim to test our hypothesis after collecting venous blood samples from adults
who were exposed to malaria as expatriate living in malaria-endemic regions.
After anonimisation of blood samples, functional immunological assays will be
performed in concert with high throughput immune-profiling approaches.
Study design
Cross-sectional
Study burden and risks
The burden and risks of participating in this study are minimal. A short
questionnaire without identifying information will be taken, along with a
single venous blood sample (32mL) that will be drawn by an experienced medical
doctor.
Geert Grooteplein 26-28
Nijmegen 6500 HB
NL
Geert Grooteplein 26-28
Nijmegen 6500 HB
NL
Listed location countries
Age
Inclusion criteria
Fifty healthy adults who have lived in malaria endemic regions for a minimum of 5 years.
Exclusion criteria
Lived in malaria endemic region for less than 5 years
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL46352.091.13 |