To perform a comprehensive analysis of the cellular infiltrate in lesional AD skin, using state of the art immunohistochemical techniques.
ID
Source
Brief title
Condition
- Allergic conditions
- Epidermal and dermal conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
We will study the number of skin infiltrating cells per square millimetre skin
in lesional AD skin, non-lesional AD skin, lesional psoriasis skin,
non-lesional psoriasis skin and skin after atopy patch testing. This includes
the analysis of:
- T cells
- B cells
- NK cells
- Dendritic cells
- Eosinophils
- Macrophages
- Mast cells
- Basophils
- Neutrophils
Secondary outcome
Not applicable
Background summary
Atopic dermatitis (AD) is a pruritic skin disease that is histologically
characterised by spongiosis (epidermal intercellular edema) and epidermal
thickening. The skin is infiltrated by a large variety of inflammatory cells,
including T cells, B cells, NK cells, eosinophils, mast cells, macrophages,
dendritic cells, basophils and neutrophils.2 Although a lot of research has
been conducted to understand the pathophysiology of atopic dermatitis, the
exact mechanism remains unknown. To understand the pathogenesis of AD, it is
essential to exactly know which immune cells are present in AD skin.
Study objective
To perform a comprehensive analysis of the cellular infiltrate in lesional AD
skin, using state of the art immunohistochemical techniques.
Study design
Observational study:
4 millimeter skin biopsies will be taken from three different groups:
- lesional versus non lesional skin biopsies will be taken from AD patients (2
biopsies per patient)
- lesional versus non-lesional skin will be taken from psoriasis patients (2
biopsies per patient)
- nonlesional versus atopy patch tested skin in AD patients (3 biopsies per
patient)
Study burden and risks
Performing a biopsy entails a slight risk of haemorrhage and infection. A small
scar at the site of biopsy will gradually fade in color. An APT will induce
erythema and pruritus with sometimes papules and/or vesicles. This reaction
gradually disappears after 72-96 hours.
The aim of this study is to investigate the presence of the different types of
infiltrating immune cells in lesional AD skin. This will gain more insight in
the pathogenesis of AD.
Heidelberglaan 100
Utrecht 3508GA
NL
Heidelberglaan 100
Utrecht 3508GA
NL
Listed location countries
Age
Inclusion criteria
1. AD group:
- age range 18 to 70 years
- AD according to diagnostic criteria of Hanifin and Rajka
- patients for APT: sensitised to aeroallergens (previously shown to have a positive APT)
2. Psoriasis group
- chronic plaque psoriasis patients, ages 18-70 years
Exclusion criteria
- topical treatment of the biopsy region with corticosteroids or tacrolimus in the two weeks prior to taking the biopsies
- treatment with systemic corticosteroids or other immunosuppressive medication in the four weeks prior to taking the biopsies
- exposure of biopsy location to (extraordinary) UV sunlight (e.g. UV-therapy, sunny holiday) in in the two weeks prior to taking the biopsies
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL43326.041.13 |