We will examine the activation status of platelets, the content of platelet granules, and activation of de novo synthesis of mitogenic proteins during and after a partial liver resection in patients with a malign or benign tumor including distant…
ID
Source
Brief title
Condition
- Hepatobiliary neoplasms malignant and unspecified
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
In this study we will examine at different timepoints during or after a liver
resection or a PPPD:
a) the activation status of platelets
b) platelet activatability
c) the content of platelet granules, and
d)t he activation of de novo synthesis of mitogenic proteins.
Secondary outcome
N/A
Background summary
De liver has a unique capacity to regenerate following surgical resection of
part of the liver. Bloodplatelets have been shown to play a crucial role in
liver regeneration after a liver resection in rodents. Also human studies
suggest an important role of platelets in liver regeneration after resection.
The precise meolecular mechanisms involved in platelet-mediated regeneration
are thusfar unclear. It appears plausible that growth factors that are stored
in platelet storage granules play a role, but it is unclear when and how these
growth factors are released during the process of regeneration. Platelet
activation is required for release of storage granule content, but it is
unknown if and when platelets are activated during the course of regeneration.
Recent studies have also shown that grwoth factors can be synthesized de novo
by platelets, but it is unknown whether this process is activated in vivo
following a liver resection.
Study objective
We will examine the activation status of platelets, the content of platelet
granules, and activation of de novo synthesis of mitogenic proteins during and
after a partial liver resection in patients with a malign or benign tumor
including distant metastasis to the liver. We aim to dissect the contribution
of liver regeneration and of the surgical procedure itself on the platelet
activation status, the content of platelet granules, and the amount of
unspliced versus spliced mRNA for the various growth factors. Our control group
consists of patients who undergo a pylorus preserving pancreaticoduodenectomy
(PPPD) for pancreatic head cancer.
Study design
Observational
Study burden and risks
In this study we will draw a blood sample at different time points during and
after surgery. This risk of the blood draw is a local hematoma. The circulating
blood volume will not be significantly affected by the blood draw.
Intraoperatively we will take a blood sample directly from the portal vein and
directly from the hepatic artery. This risk associated with these blood draws
is very small and will not have any influence on operation procedure and
outcome. Earlier studies have shown that our HPB team is able to draw blood
from these vessels safely. Worst case scenario is a bleeding, which will be
arrested by a single suture.
Hanzeplein 1
Groningen 9713 GZ
NL
Hanzeplein 1
Groningen 9713 GZ
NL
Listed location countries
Age
Inclusion criteria
Sutdy group: (extended) right hemi-hepatectomy for a malign or benign tumor including distant metastasis the liver
For the control group: pylorus preserving pancreaticoduodenectomy for pancreas head cancer.
Exclusion criteria
1) age: patients younger than 18 and older than 65 years of age;
2) patients who use (selective)serotonin reuptake inhibitors (SSRI);
3) patients with arterial thrombosis in their medical history;
4) patients who use anticoagulants such as vitamin K antagonists, aspirin, and plavix;
5) patients with liver cirrhosis.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL36835.042.11 |