1. Evaluation of differences in free fatty acid (FFA) profiles of several adipose tissue depots, expressed as FFA ratios, between metabolically healthy obese subjects and metabolically unhealthy obese subjects (e.g. subjects with metabolic syndrome…
ID
Source
Brief title
Condition
- Glucose metabolism disorders (incl diabetes mellitus)
- Arteriosclerosis, stenosis, vascular insufficiency and necrosis
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
1. Free fatty acid ratios
Triglycerides (TG), poly unsaturated fatty acids (PUFA) and total unsaturated
fatty acids (TUFA) will be measured with spectroscopy.
The PUFA/TG, TUFA/TG and the PUFA/TUFA ratio will be calculated after
standardization of the measurements with respect to voxelsize and watercontent.
2. Serum triglycerides vs adipose tissue triglycerides
The change in concentration of triglycerides in serum (peripheral blood) will
be compared to the change in concentration of triglycerides in the adipose
tissue measured with spectroscopy, both before and after an oral fat load.
Secondary outcome
Not applicable
Background summary
Adipose tissue dysfunction can be diagnosed with 1 (H)-MR-spectroscopy,
quantifying free fatty acids. Moreover, differences can be encountered in the
free fatty acid distribution between lean and obese persons and between persons
with and without characteristics of the metabolic syndrome. The development of
adipose tissue dysfunction is partly driven by the amount of adipose tissue
present in the body. To what extent diet, exercise and other environmental
factors play a role in the development, maintenance and amelioration of adipose
tissue dysfunction is topic of current research
Study objective
1. Evaluation of differences in free fatty acid (FFA) profiles of several
adipose tissue depots, expressed as FFA ratios, between metabolically healthy
obese subjects and metabolically unhealthy obese subjects (e.g. subjects with
metabolic syndrome) using 1 (H)-MR-spectroscopy. (MRS)
2. Evaluation of differences in FFA profiles of several adipose tissue depots
(expressed as FFA ratios) before and after an oral fat load in metabolically
healthy obese subjects and metabolically unhealthy obese subjects (e.g.
subjects with metabolic syndrome) using 1 (H)-MR-spectroscopy.
Study design
A cross-sectional study with 2 MRS measurements per individual, one fasting and
1 after ingestion of an oral fat load.
Study burden and risks
Participants are asked to refrain from high fat foods for 3 days before MRS
measurements take place, and to ingeste an oral fat load on the day of the MRS
measurements. Per participant there will be 2 MRS measurements and 3
bloodsamples will be drawn. No contrast will be given during the MRS.
Therefore, we consider this study to be low-risk.
Heidelberglaan 100
Utrecht 3584 CX
NL
Heidelberglaan 100
Utrecht 3584 CX
NL
Listed location countries
Age
Inclusion criteria
1. Between ages of 18 and 70 years.
2. Male or female
3.a. Metabolic syndrome group: BMI *25 kg/m2 and presence of the metabolic syndrome.
3.b. No metabolic syndrome group: BMI *25 kg/m2 and absence of the metabolic syndrome.
Exclusion criteria
1. Known cardiovascular disease
2. Known renal, liver or pulmonary disease
3. Diabetes mellitus
4. Use of medication (except for oral anticonceptives, proton pump inhibitor*s, inhalation medication or topical unguents)
5. Pregnancy or lactation
6. Claustrophobia
7. Waist circumference too large to fit MRI (>200cm)
8. Metallic devices in the body of the participant
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL41412.041.12 |