To examine the impact of an exercise bout on the ability to protect endothelial damage in response to ischaemia reperfusion injury in healthy humans. A secondary objective is to explore the potential difference in efficacy to prevent ischaemia…
ID
Source
Brief title
Condition
- Coronary artery disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Change in endothelial function (measured with flow mediated dilation) after
ischaemia reperfusion injury (induced by 20 minutes ischemia) with and without
precedence of an acute bout of moderate-intensity endurance or (isocaloric)
high-intensity interval exercise.
Secondary outcome
nitrite/nitrate in plasma
Background summary
Exercise training has strong cardioprotective effects in asymptomatic subjects
as well as those with established cardiovascular risk and/or disease.
Ischemia-reperfusion (IR) represents a significant and harmful stimulus for
tissues, including the myocardium and the vascular endothelium. Recent studies
have found preliminary evidence that exercise training is associated with a
reduced endothelial IR-injury, which may partly contribute to the protective
effects of exercise training. Whether also acute bouts of exercise possess the
ability to prevent endothelial IR injury in humans in vivo is currently unknown.
Recent studies have demonstrated that different types of exercise training
possess different effects, with high-intensity interval training leading to
superior effects on physical fitness and the vasculature compared to
*traditional* moderate-intensity exercise. Accordingly, different types of
exercise may also have a distinct impact on the ability to prevent endothelial
IR injury. This is supported by the observation that high-intensity interval
training (4 x 4-min high-intensity exercise) has remarkable similarities with
ischaemic preconditioning (4 x 5-min ischemia); i.e. a validated and successful
method to prevent endothelial IR-injury.
Study objective
To examine the impact of an exercise bout on the ability to protect endothelial
damage in response to ischaemia reperfusion injury in healthy humans. A
secondary objective is to explore the potential difference in efficacy to
prevent ischaemia reperfusion injury between high-intensity interval training
and traditional moderate-intensity exercise.
Study design
within-subject cross-over study
Intervention
A bout of traditional moderate-intensity exercise and high-intensity interval
exercise
Study burden and risks
Non-invasive cuff occlusion is used to examine endothelial function (5-minute
ischaemia) and produce the stimulus that induces ischaemia-reperfusion injury
(20-minute ischaemia). Cuff inflation is non-invasive and not associated with a
health risk for the subject. Also exercise is not associated with a significant
health risk in our participants. Blood will be drawn three times per testing
day for ex-vivo analysis of the NO-pathway. The volunteers will not benefit
directly from participating in this study.
Philips van Leijdenlaan 15
Nijmegen 6525 EX
NL
Philips van Leijdenlaan 15
Nijmegen 6525 EX
NL
Listed location countries
Age
Inclusion criteria
- Healthy volunteers >18 years
- Written informed consent
Exclusion criteria
-Presence of an absolute contra-indication for the performance of exercise (see also SOP Inspanningstest Department of Physiology):
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL42390.091.12 |