To describe the composition of the BMZ along the interfollicular and follicular epidermis of perilesional HS skin and to investigate whether the expression of these proteins differs from normal skin of healthy control patients. Additionally, a…
ID
Source
Brief title
Condition
- Skin appendage conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
To determine with direct immunofluorescence (DIF) whether there is a difference
in expression of the BMZ components BP180, collagen type VII, laminine-332 and
integrine*4 at the interfollicular epidermis and hair follicles between HS
patients and healthy controls. Additionally, a PAS-staining of the skin samples
will be performed to compare the degree of positivity between perilesional HS
skin and normal skin from healthy controls. Finally, the morphology of the
normal hair follicle localized on apocrine gland bearing skin will be
described.
Secondary outcome
Not applicable.
Background summary
Hidradenitis suppurativa (HS) is a chronic inflammatory skin disease that
mainly occurs on the apocrine gland bearing skin. The pathogenesis of HS is
largely unknown but several studies have shown that the initiating event takes
place at the hair follicle. A recent study demonstrated a lack of PAS-staining
positivity at the sebofollicular junction in perilesional skin of patients with
HS. This lack of PAS-positivity might be a reflection of a structural defect in
the follicular basal membrane zone (BMZ) in HS. This weakness in the BMZ could
induce the release of hair follicle contents into the dermis that subsequently
trigger an inflammatory reaction. We hypothesize that this weakness might be
caused by a diminished presence of one or more important components of the
basal membrane zone at specific parts of the hair follicle, including BP180,
laminine-332, intergrine*4 and collagenVII.
Study objective
To describe the composition of the BMZ along the interfollicular and follicular
epidermis of perilesional HS skin and to investigate whether the expression of
these proteins differs from normal skin of healthy control patients.
Additionally, a hematoxylin and eosin (HE) staining will be performed on skin
biopsies obtained from healthy volunteers to describe the normal morphoogy of
the hair follicle.
Study design
Observational study.
Study burden and risks
Skin samples of HS patients will be obtained during surgery at the armpits or
groins for HS as part of the medical treatment. Normally, the removed skin
during this surgery is thrown away. However, when patients have given informed
consent for participation in the study, biopsies will be taken from this
removed skin. These biopsies will be anonymously stored until a DIF and
PAS-staining is performed. No extra skin will be removed during surgery for the
purpose of this study so the patient will not experience any burden from
participating and there are no risks related to participation. However,
participation will not be of benefit for the patient. To draw any conclusions
on the results from the staining of skin biopsies of HS patients, it is
necessary to compare these with stainings from healthy control skin. Skin of
healthy volunteers will be obtained by means of a 4mm punch biopsy under local
anaesthesia. It is a generally safe procedure with minimal burden to the
patient. Possible complications of bruising, bleeding, infection and scarring
rarely occur.
Hanzeplein 1
Groningen 9700 RB
NL
Hanzeplein 1
Groningen 9700 RB
NL
Listed location countries
Age
Inclusion criteria
Patients with HS who have been planned for surgical treatment of HS, aged 18-50 years.;Healthy volunteers with no skin diseases located in the armpits, aged 18-50 years
Exclusion criteria
Lack of informed consent
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL47372.042.14 |