The results of this research will elucidate the differences in oxygen tension and further effects for both adipose tissue depots in obese, insulin resistant woman. A recent study showed an increased oxygen tension in obese insulin resistant subjects…
ID
Source
Brief title
Condition
- Glucose metabolism disorders (incl diabetes mellitus)
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Differences in oxygen tension and further effects for both adipose tissue
depots in obese, insulin resistant woman will be investigated. We will perform
our experiments in the abdominal and femoral adipose tissue depots and also
monitor the blood flow in both depots. Next to this we will also determine the
local inflammation.
The primary parameter is:
- oxygen tension in adipose tissue
Secondary outcome
Secundary parameters are:
- adipose tissue blood flow
- insulin sensitivity
- inflammation markers in adipose tissue (IL-6, TNF-alfa, CD68, leptin,
adiponectin, MCP-1, MIF1, PPAR-gamma, CD34 mRNA expressie)
- bodyweight, body mass index (BMI) and body composition
- whole body adipose tissue distribution (DEXA scan)
- adipocyte size
- plasma glucose concentration
- plasma insulin concentration
- glucose tolerance
- FFA, triacylglycerol, glycerol concentrations
- bloodpressure
We will also isolate cells from the biopsies and expose them to different
concentrations oxygen. With this experiment we will investigate the effects of
oxygen tension on adipocyte metabolism and inflammation.
Background summary
Research showed that adipose tissue is active as an organ and plays an
important role in the metabolism. Individuals with obesity and type 2 diabetes
have a disturbed adipose tissue. Convincing evidence showed that this
disturbance is important in the development of insulin resistance (reduced
effect of the hormone insulin) and type 2 diabetes.
Recently, we have shown that subjects with overweight/obesity and insulin
resistance have an increased oxygen tension. There are two major adipose tissue
depots: abdominal and femoral (upper leg). According to literature these depots
have different characteristics, and the femoral depot is suggested to have a
more beneficial effect. We expect the oxygen tension to play a part in this
difference and that through our study we can characterize both depots.
Study objective
The results of this research will elucidate the differences in oxygen tension
and further effects for both adipose tissue depots in obese, insulin resistant
woman. A recent study showed an increased oxygen tension in obese insulin
resistant subjects.
This research will contribute to an increased insights in the existence and
development of insulin resistance and type 2 diabetes. This could lead to
improved prevention and treatment of insulin resistance, type 2 diabetes and/or
related diseases.
Study design
Candidate-subjects will be selected through a screening, which will show if an
individual is suitable for this research. Screening will be performed with more
individuals than the actual study population. The results obtained from the
screening will be used to evaluate if an individual is suitable for this
research. Participation in the screening will not necessarily result in
participation in the research.
If an individual is suitable for this research they will have to visit the
university 3 times (see "tijdsinvestering" page 7 of the
"proefpersooninformatie", dutch only) for measurements leading to answers on
our research questions.
Intervention
n/a
Study burden and risks
All measurements are easy to cope with, without additional related risks. The
insertion of the canule can incidentally cause a hemorrhage. The biopsies will
be taken under local anesthesia by an experienced individual. A hemorrhage can
occur at the place of the biopsy and this area can stay sensitive for several
days. The biopsies will leave a small scar (approximately 3 mm).
universiteitssingel 50
Maastricht 6229 ER
NL
universiteitssingel 50
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
- Woman with overweight/obesity (body mass index >= 28 kg/m2) and a disturbed glucose metabolism (IFG: 6.1-7.0 mmol/l (or 5.6-7.0 mmol/l and a family history of diabetes) and/or IGT: 2h plasma glucose during OGTT 7.8-11.1 mmol/l)
- postmenopausal
- Age: 40-65
- Non smoking
- A stable bodyweight for at least 3 months (no change in bodyweight: < 3kg)
Exclusion criteria
- diabetes mellitus
- cardiovascular diseases
- cancer
- asthma or bronchitis
- liver and/or kidney disease (determined based on ALAT and creatinine levels, respectively)
- a disease with a life expectancy < 5 years (will be questioned for each individual)
- alcohol and/or drug abuse (alcohol consumption > 15 units/week)
- plans to lose weight (subjects will be asked if they have weight loss plans (e.g. to increase their physical activity level or change diet): a positive answer will lead to exclusion)
- participation in sport activities for more than 3 hours/week
- Usage of higher doses anti-oxidants (vitamin A, C, E, beta-carteen; standard multi-vitamin supplements are allowed when the concentrations are less than: 800ug/day Vit A, 60mg/day Vit C, 10mg/day Vit E and 400ug/day beta-caroteen)
- use of medication with an effect on the glucose metabolism or inflammation
- not able to understand the subject information
- smoking
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL46328.068.13 |