Gut microbiotica composition and postprandial effect on endotoxemia in obese healthy males and obese insulin resistant males

There is a worldwide epidemic of obesity, which is a major risk factor for the
development of common medical conditions such as type 2 diabetes, dyslipidemia
and subsequent cardiovascular disease. Novel insights show that gut microbiota
composition altered by diet takes a great part in the development of
postprandial endotoxemia, which is thought to be related to the inflammatory
process in the adipose tissue. These inflammatory changes, such as the level of
influx of CD68+ macrophages into adipose visceral, can trigger a local and
systemic inflammatory response with disturbed adipocyte lipolysis, which in
turn induces insulin resistance in other tissues such as liver and muscle.
Therefore we aim to investigate the feces composition and the postprandial
effect on endotoxemia (e.g. LPS and Ralstonia) in plasma in lean healthy males
and obese insulin resistant males

The aim for this study is to investigate feces composition and postprandial
effect, as done with oral fat loading test (OFLT), on endotoxemia (e.g. LPS and
Ralstonia levels) in plasma as assessed with qPCR.

This is an observational study with an OFLT.

Subjects will visit the hospital twice. During the first visit, informed
consent will be signed and patients are screened for eligibility. When subjects
can be included in the study they are asked to visit the AMC for the second
time. There the collection of the patients* morning stool will be handled.
Hereafter the oral fat loading test will take place to asses postprandial
endotoxemia (e.g. LPS and ralstonia) in plasma. This study does not have
specific advantages for the subjects. The results of this observational study
may help us to understand the role of gutmicrobiota in the feces in the
development of postprandial endotoxemia. Total study duration for each subject
will be about 10 hours. Fat loading test is a routine test in our department
which requires repetitive blood sampling from an indwelling venous catheter
after the consumption of cream and Vitamin A. This test does not cause any side
effects. We believe the information gathered from this study outweighs the
burden of this investigation.