To investigate the impact of 7 days of bed-rest on skeletal muscle mass, strength and whole-body insulin sensitivity in healthy, young men.
ID
Source
Brief title
Condition
- Other condition
- Metabolism disorders NEC
- Muscle disorders
Synonym
Health condition
muscle metabolism
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Quadriceps muscle cross sectional area (CSA)
Secondary outcome
Whole body insulin sensitivity, whole body lean mass, leg lean mass, lower back
muscle CSA, muscle strength, type I and II muscle fiber CSA and SC content,
mRNA and protein expression of key genes involved in the regulation of muscle
mass.
Background summary
Admittance to hospital generally leads to a period of whole body disuse (i.e.
bed-rest). Prolonged bed-rest causes substantial skeletal muscle loss and
subsequent negative health consequences. However, the average length of stay in
hospital for acute illness is approximately one week. It is currently unknown
what the impact only 7 days bed-rest has on skeletal muscle mass and metabolic
health.
Study objective
To investigate the impact of 7 days of bed-rest on skeletal muscle mass,
strength and whole-body insulin sensitivity in healthy, young men.
Study design
Single group intervention study with pre and post intervention testing.
Intervention
Seven days of bed-rest.
Study burden and risks
The risks involved in participating in this experiment are minimal. Muscle
biopsies will be taken through a small (5 mm) incision, following local
anesthetics of the skin and muscle fascia, and will heal completely. Muscle
biopsies will only be obtained by an experienced physician. Seven days of
bed-rest will impair subject*s mobility for this period but they will be
monitored constantly by researchers. There is a risk of DVT, which will be
minimized by performing exercises to activate the calf muscle three times
daily. Additionally, on days 0, 2, 4, 6 and 7 of bed-rest, additional blood
will be collected to determine D-dimers and other coagulation factors, to
ensure an accurate and quick response in case of the development of thrombosis.
The expected loss of muscle mass and strength following bed-rest will be
rapidly (<4 weeks) regained due to the inclusion of young, healthy volunteers.
Universiteitssingel 50 - room 2.208
Maastricht 6229 ER
NL
Universiteitssingel 50 - room 2.208
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
- Male
- Aged from 18-35 years
- 18.5 < BMI < 30 kg/m2
- Recreationally active (performing non-competitive physical exercise at least one time per week for minimally 60 minutes)
Exclusion criteria
- Smoking
- Performing regular resistance training (3+ times per week, carrying out progressive training) in the previous 6 months
- Hypertension (according to WHO criteria) [45] and/or cardiovascular disease
- Any back/leg/knee/neck/postural complaints
- Use of any prescribed medication
- Type 2 diabetes mellitus
- Any family history of thrombosis
- All co-morbidities interacting with mobility and muscle metabolism of the lower limbs
(e.g. arthritis, spasticity/rigidity, all neurological disorders and paralysis).
- Myocardial infarction within the last 3 years
- Use of anti-coagulants
- A history of deep vein thrombosis (DVT) in the leg
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| ClinicalTrials.gov | NCT02109380 |
| CCMO | NL48569.068.14 |