Investigate whether differences of the thermostat set point between the sexes might be responsible for BAT differences between men and women. Secondly, we investigate whether women have a different glucose uptake during cold exposure than men.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
thermoregulatie
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Shivering temperature
Secondary outcome
Glucose tolerance
Background summary
Obesity is a significant and increasing health problem in modern society. Brown
adipose tissue (BAT) in adult humans might have the potential to increase
energy expenditure by the process of thermogenesis and is thereby able to
reduce body weight. BAT can be stimulated by cold exposure but this is not
applicable to the general population. In order to find other methods to
activate BAT, we need a better understanding of BAT activity regulation.
Previous studies have shown that in general, prevalence of activated BAT is
higher in women than in men. Different regulation of BAT activity might be due
to a different set point of the thermostat in the hypothalamus.
Study objective
Investigate whether differences of the thermostat set point between the sexes
might be responsible for BAT differences between men and women. Secondly, we
investigate whether women have a different glucose uptake during cold exposure
than men.
Study design
The temperature set point will be determined by measuring the shivering
temperature of subjects. Subjects are cooled using cooling vests connected to a
cooling device, while muscle activity is measured by electromyogram (EMG).
Shivering temperature is considered as the temperature (of the vest) at which
the EMG starts to show muscle activity. Oral glucose tolerance tests are
performed without and during cold exposure at 2 C above the shivering
temperature in a crossover design. Differences in glucose tolerance between
sexes and temperature are analyzed.
Intervention
Cold exposure
Study burden and risks
During determination of shivering temperature subjects will be exposed
increasingly lower temperatures (minimally 10 C) to cold for approximately 2
hours. For the glucose tolerance test, subjects are asked to fast overnight and
to drink a high concentration glucose potion. During the test, 8 finger pricks
are needed to determine capillary blood glucose levels over 150 min. The test
will be carried out twice: once during cold exposure to 2 C above the shivering
temperature and at room temperature.
There will be no direct benefit of participation for the subjects.
The possibility exists that previously undiagnosed (beginning) diabetes will be
identified.
Wytemaweg 80
Rotterdam 3015CN
NL
Wytemaweg 80
Rotterdam 3015CN
NL
Listed location countries
Age
Inclusion criteria
age between 16 and 35 years
Exclusion criteria
BMI < 18.5 or BMI > 30
Diabetes Mellitus
Beta-blocker use
If female, pregnancy or breast feeding
Drug or alcohol dependence or abuse
Participation in a trial of an experimental drug or device within 60 days prior to screening
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL53869.078.15 |