To study alteration in platelet function and RNA transcripts during and after a Gram positive and Gram negative infections.
ID
Source
Brief title
Condition
- Bacterial infectious disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
1) Platelet transcriptome during and after bacteraemia. 2) Platelet function
and platelet-leukocyte interaction
Secondary outcome
3) Platelet granule content (proteomics) 4) microparticle levels and phenotype
during disease.
Background summary
: Infectious diseases are associated with an increased risk for cardiovascular
diseases (CVD). The mechanisms underlying this association are not fully
elucidated. The risk for CVD following gram positive bacterial infections, such
as caused by Streptococcus pneumonia or Staphylococcus aureus seems higher than
during gram negative bacterial infections. Clinical studies have shown that the
increased CVD for CVD also persists for a prolonged period of time.
Platelets are key cells in acute cardiovascular events but also in the
progression of atherosclerosis. Platelets can recognize microbes and influence
leukocyte function via a direct platelet-leukocyte interaction. Preliminary
data from our laboratory showed that acute gram positive bacteremia is
associated with more platelet activation and platelet-leukocyte interaction
than gram negative bacteremia. How long these changes persist is unknown. The
life span of a platelet is only 10 days, but infections may influence
megakaryocytes. Platelets possess a wide array of RNA transcripts which can be
altered during a wide array of diseases. The hypothesis that forms the basis of
this study proposal is that a gram positive infection causes long term changes
in platelet function and RNA transcript expression.
Study objective
To study alteration in platelet function and RNA transcripts during and after a
Gram positive and Gram negative infections.
Study design
A prospective observational study.
Study burden and risks
Participation in this study will not influence standard care. The study
involves a maximum of one extra visit to the outpatient clinic and a maximum of
three extra venipunctures. If possible the blood collection will be done during
a venipuncture planned for regular care. There is a maximum of 159ml for the
whole study procedure (6 weeks)
Geert Grooteplein-Zuid 10
Nijmegen 6525 GA
NL
Geert Grooteplein-Zuid 10
Nijmegen 6525 GA
NL
Listed location countries
Age
Inclusion criteria
• >18 years
• Following infections:
o Positive blood culture pathogenic Gram positive bacteria (eg. Staphylococcus aureus (at least 10 with S. aureus), Streptococci)
o Positive blood culture with a pathogenic Gram negative bacteria (eg. E. coli (at least 10 with E. coli), K. pneumoniae)
• Expected duration of in-patient care of at least 3 days.
• Signed informed consent
Exclusion criteria
• Use of platelet function inhibitors (except aspirin)
• Antibiotic therapy for longer than 72 hours
• Myocardial infarction or stroke in the previous year
• An active hematologic or solid malignancy
• Terminal renal failure requiring dialysis
• Insulin dependent diabetes mellitus
• Solid organ transplant requiring anti-inflammatory medication
• Use of anti-inflammatory agents such as prednison (>7.5mg) or use of targeted biological agents (eg. TNF-blockers, monoclonal antibodies) with the exception of NSAIDs
• Micro-organisms that are well known contaminants in a blood culture (e.g. coagulase negative staphylococcus)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL57239.091.16 |