De objective of this study is to analyse the effect of exercise on the levels of Ara h6 in the blood after peanut consumption. Next to this we would like to know whether these levels found are in associated with the measure of intestinal…
ID
Source
Brief title
Condition
- Gastrointestinal conditions NEC
- Allergic conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The primary outcome is the serum Ara h6 level after intake of peanuts with and
without exercise
Secondary outcome
The secondary outcome is the lactulose/rhamnose ratio in plasma after intake of
the dual sugar solution with and without exercise
Background summary
Since the exponential increase in the prevalence of food allergies, the number
of studies in this field have also increased exponentially. In order to support
ths research, well-defined methods for measuring allergens are needed.
Recently, we developed an ELISA method for the detection of Ara h6 in blood
after peanut consumption.
Exercise is known to increase intestinal permeability and it is hypothesized
this could possibly lead to increased levels of (partly) digested dietary
proteins, thus allergens also, could end up in the bloodstream.
Study objective
De objective of this study is to analyse the effect of exercise on the levels
of Ara h6 in the blood after peanut consumption. Next to this we would like to
know whether these levels found are in associated with the measure of
intestinal permeability, which we can measure with the double-sugartest.
Study design
In this study with a crossover design subjects are requested to come to the
university while fasted overnight to consume peanuts and a dual sugar solution.
On the second test day these consumptions will be followed by a cycle test
consisting of steady state cycling for 1 hour at 75% Wmax (Which will be assed
with a maximal cycling test during the pre-testing). Prior to (baseline) and
30-60-90-120-240min after intake of peanuts and dual sugar solution, blood will
be collected via a venflon cannula. At the end of each test day, the subjects
will receive lunch.
Intervention
Intake of 100grams of peanuts and a dual sugar solution (5gr lactulose and 1 gr
rhamnose), whether or not followed by a cycling test (1 hour 75% Wmax)
Study burden and risks
Subjects need to fill out a screening questionnaire and height and weight are
measured. Next to this a fasted blood sample will be drawn to test for an
inhibitory matrix effect in the Ara h6 ELISA method. Furthermore they need to
complete a maximal cycling test during pre-testing, and 1 hour of steady state
cycling at 75% of their Wmax during the second test day.
The exercise intensity is adapted to individual capacity and could at most lead
to some muscle fatigue and soreness afterwards. The subjects have to keep some
dietary guidelines. Blood withdrawal could lead to minor bruising and stiffness
of the arm, and is minimalized by using a venflon canula. Per test day, a total
of 72mL blood will be samples at regular intervals during approximately 5
hours, making 144mL in total.
Bornse Weilanden 9
Wageningen 6708 WG
NL
Bornse Weilanden 9
Wageningen 6708 WG
NL
Listed location countries
Age
Inclusion criteria
- 20-35 year old male or female
- Body mass index (BMI) 23-27.5 kg/m2
- Suitable veins for blood sampling
- No inhibitory matrix effect in Ara h6 ELISA method
- Able to cycle 1h on ergometer
Exclusion criteria
- History of peanut allergy
- Known symptoms of immune disease, such as diabetes, gastritis, and coeliac disease.
- Pregnant or lactating
- Known symptoms of intestinal disease, such as Crohn*s Disease, ulcerative colitis, and irritable bowel syndrome.
- Smoking
- Use of hard drugs
- Use of specific medicines:
o Chronic use of NSAIDs: aspirins, ibuprofen, etc.
o Drugs having an effect on gastric and/or intestinal function and motility, including antidepressants.
- Participation in other scientific studies
- Blood donation during the last six weeks before the start and during the study
- Employed at Human Nutrition, Human and Animal Physiology, or Consumer Science and Health(FBR) or performing an internship/thesis at any of these places.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL57339.081.16 |