To assess whether the inhibitory effect of CK on platelet aggregation can be reversed in vitro.
ID
Source
Brief title
Condition
- Coagulopathies and bleeding diatheses (excl thrombocytopenic)
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Inhibition of the effect of creatine kinase on platelets in vitro
Secondary outcome
not applicable
Background summary
We have previously shown that creatine kinase, the enzyme that utilizes ADP and
phosphocreatine to rapidly regenerate ATP, inhibits platelet aggregation in
vitro (Horjus 2014). This mechanism may lead to higher bleeding risk in persons
with high activity of creatine kinase. Now, we want to assess whether this
effect of CK can be reversed in vitro.
Study objective
To assess whether the inhibitory effect of CK on platelet aggregation can be
reversed in vitro.
Study design
This is an observational study in vitro using human venous blood. The in vitro
study will be conducted at the Department of Clinical Chemistry, AMC,
Amsterdam. We will assess, after informed consent, in venous blood in vitro,
CK, creatinine, glucose, ASAT, ALAT, thrombocyte count, coagulation tests
(aPTT, PT). In addition, we will assess whether the inhibitory effect of CK on
platelet aggregation can be reversed in vitro.
Volunteers will come to the hospital after an overnight fast. After signing of
the informed consent and a short health questionnaire, 50 ml of venous blood
will be drawn. Volunteers receive the results of the blood test the next
working day by telephone. If we find any abnormality, the family doctor will be
informed as well.
Study burden and risks
There is no benefit for the participant, while the risk is considered to be
negligible
Meibergdreef 5
Amsterdam 1105AZ
NL
Meibergdreef 5
Amsterdam 1105AZ
NL
Listed location countries
Age
Inclusion criteria
Healthy volunteers 18-60 years old not using any medication
Exclusion criteria
A potential subject who meets any of the following criteria will be excluded from participation in this study: any known disease as assessed by history taking or the obligatory use of (prescription) drugs.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL57523.018.16 |