We here wish to apply new advancements in multi-color flow cytometry for a detailed and sensitive examination of mast cells in peripheral blood of patients with SM.
ID
Source
Brief title
Condition
- Haematopoietic neoplasms (excl leukaemias and lymphomas)
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
To study the reliable identification of mast cells in peripheral blood of
patients with systemic mastocytosis and investigate if numbers and/or phenotype
(expression of aberrant markers) differ from healthy controls.
Secondary outcome
To study numbers and phenotype of other leukocyte subsets (with an emphasis on
IgE+ B cells) in peripheral blood of patients with SM compared to healthy
controls.
Background summary
Systemic Mastocytosis (SM) is a disease characterized by the accumulation of
mast cells in various tissues, mainly in the skin and in the bone marrow.
Manifestations of the disease are largely provoked by the resultant increase in
mast cell-derived mediators which have a variety of local and systemic effects.
Diagnosis is based on a combination of major and minor criteria. In any case,
investigation of the bone marrow (BM) is a hallmark in the diagnosis of SM,
either for analysis of mutation status or flow cytometric identification of
aberrant mast cells. Currently, detection of mast cells in peripheral blood has
not been possible.
Study objective
We here wish to apply new advancements in multi-color flow cytometry for a
detailed and sensitive examination of mast cells in peripheral blood of
patients with SM.
Study design
Cross sectionel study at the outpatient clinic of the department of Immunology
where at one time an extra tube of blood will be drawn from patients diagnosed
with systemic mastocytosis.
Study burden and risks
There is a minimal burden for patients. Patients are already undergoing a
venapunction for scheduled follow up different blood values, only an extra tube
of blood needs to be drawn.
Wytemaweg 80
Rotterdam 3015 CE
NL
Wytemaweg 80
Rotterdam 3015 CE
NL
Listed location countries
Age
Inclusion criteria
Older than 18 years
Diagnosed with systemic mastocytosis according to WHO criteria
Informed written consent
Exclusion criteria
Younger than 18 years
No informed consent
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL49666.078.14 |