To assess the anabolic properties of free amino acids and milk protein.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
To assess the anabolic response following protein ingestion
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
muscle bound L-[ring-2H5]-phenylalanine, L-[1-13C]-phenylalanine, and
L-[1-13C]-leucine enrichments (in MPE)
plasma L-[ring-2H5]-phenylalanine and L-[1-13C]-leucine enrichments (in MPE)
Secondary outcome
Plasma amino acid, glucose en insulin concentrations, "whole-body" metabolism
Background summary
Muscle tissue consists of proteins. These proteins are built up of small
building blocks: amino acids. By consuming enough protein in our diet, we make
sure that the body is provided with enough amino acids to facilitate muscle
growth (also called muscle protein synthesis). Currently, it is known that
different protein sources can have different effects on muscle growth.
Although, it is unknown whether free amino acids can stimulate muscle growth
more than regular milk protein.
Study objective
To assess the anabolic properties of free amino acids and milk protein.
Study design
Parallel group, randomized, double blind.
Intervention
Assess the muscle protein synthetic response after ingestion of a protein
beverage.
Study burden and risks
The burden and risks involved in participating in this experiment are small.
Insertion of the catheters in a vein is comparable to a normal blood draw and
the only risk is a small local hematoma. Muscle biopsies will be obtained under
local anaesthesia by an experienced physician, but may cause some minor
discomfort. The discomfort is comparable to muscle soreness or the pain one has
after bumping into the corner of a table. During the experimental trial 20
blood samples (210mL total) will be obtained. The total amount of blood
collected is less than half the amount of a blood donation and will be
completely restored in approximately 1 month. The stable isotope amino acids
tracers that will be infused intravenously during the experimental trial are
produced according to GMP standards and are safe for human use.
Univerisiteitssingel 50
Maastricht 6229 ER
NL
Univerisiteitssingel 50
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
1) Aged 18-35 y
2) BMI 18.5-27.5 kg/m2
3) Healthy, recreationally active, young adults
Exclusion criteria
1) Allergies to milk protein
2) Lactose intolerance
3) PKU disease
4) Smoking
5) Diagnosed diabetes
6) Diagnosed metabolic or intestinal disorders
7) A history of neuromuscular problems
8) Any medications known to (or may) affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications)
9) Participation in structured resistance exercise program
10) Pregnant
11) Hormone replacement therapy
12) Third generation oral contraceptives
13) Strict vegetarian
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL63767.068.17 |
| Other | Protocol will be registered at NTR after approval by METC |