To compare the plasma amino acid responses after the digestion of a milk protein powder with varying levels of protein glycation in healthy young men.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
eiwitvertering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Peak plasma lysine concentration
Secondary outcome
Plasma amino acid, insulin-, and glucose responses.
Background summary
Protein intake is an essential stimulus for tissue replacement/growth in
infants, as well as a strong regulator of muscle protein anabolism in athletes.
The anabolic effect of protein ingestion is mainly determined by the plasma
amino acid response after ingestion. Although some literature suggests that
glycation of proteins, which can occur during commonly applied milk processing
procedures, might attenuate the digestibility of a dairy product, this has not
yet been extensivly assessed in humans.
Study objective
To compare the plasma amino acid responses after the digestion of a milk
protein powder with varying levels of protein glycation in healthy young men.
Study design
Dubble blind cross-over study with 3 interventions
Intervention
Subjects will perform three experiments in a double-blind, randomized order:
- 40 g of milk protein with 5% glycation level in 600 mL water
- 40 g of milk protein with 20% glycation level in 600 mL water
- 40 g of milk protein with 50% glycation level in 600 mL of water.
After ingestion, blood samples will be taken at regular intervals during a 6
hour period.
Study burden and risks
The risks involved in participating in this experiment are minimal. Insertion
of the catheters in a vein is comparable to a normal blood draw and the only
risk is a small local hematoma. The milk protein supplements are produced
according to GMP standards and are safe for human use.
Universiteitssingel 50
Maastricht 6229 ER
NL
Universiteitssingel 50
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
- Males
- Aged between 18-35 years
- Healthy, recreationally active (participating in recreational sports activities <= 3 times per week)
- BMI < 30 kg/m2
- No physical limitations (i.e. able to perform all activities associated with daily living in an independent
Exclusion criteria
- Smoking
- Allergies to milk proteins
- Allergies to soya products
- Musculoskeletal disorders
- Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications).
- Chronic use of gastric acid suppressing medication or anti-coagulants- Unstable weight over the last three months
- Pathologies of the gastrointestinal tract
- Strictly vegetarian diet
- Blood donation in the past 2 months
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL62254.068.17 |
| OMON | NL-OMON29239 |