To compare the digestion and absorption of micellar casein versus calcium-caseinate and sodium-caseinate with enzymatic cross-linking in healthy young men.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
eiwitvertering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Plasma amino acid response
Secondary outcome
Plasma insulin-, and glucose responses
Background summary
Protein intake is a strong stimulus for muscle protein anabolism, both at rest
and after exercise. The anabolic effect of a protein supplement is mainly
determined by the digestion and absorption kinetics of the ingested protein
source. It is suggested that a slowly digested protein source is superior over
a rapidly digested protein source during periods of limited food intake. In
accordance, we have previously shown that the intake of casein, which is a
slowly digested protein, increases muscle protein synthesis during the night.
Casein can be provided either as micellar casein that clots as it enters the
acid milieu of the stomach, or as a caseinate that remains solid. If casein
clots or remains in solution may have an effect on its digestion and absorption
kinetics and subsequent muscle protein synthesis rates. To determine the
optimal composition of a casein supplement, the differences in digestion and
absorption of casein versus calcium-caseinate and sodium-caseinate with
enzymatic cross-linking should be examined first.
Study objective
To compare the digestion and absorption of micellar casein versus
calcium-caseinate and sodium-caseinate with enzymatic cross-linking in healthy
young men.
Study design
Randomized, double-blinded, crossed-over experiment
Intervention
Subjects will be randomly assigned to three experiments:
- a test beverage of 600 mL containing 40 g micellar casein in water,
- a test beverage of 600 mL containing 40 g calcium-caseinate in water,
- a test beverage of 600 mL containing 40 g sodium-caseinate with
enzymatic-cross linking.
After ingestion, blood samples will be taken at regular intervals during a 6
hour resting period.
Study burden and risks
The risks involved in participating in this experiment are minimal. Insertion
of a catheter in a vein is comparable to a normal blood draw and the only risk
is a small local hematoma.
Universiteitssingel 50
Maastricht 6229 ER
NL
Universiteitssingel 50
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
- Males
- Aged between 18-35 years
- Healthy, recreationally active (participating in recreational sports activities <= 3 times per week)
- BMI < 25 kg/m2
- No physical limitations (i.e. able to perform all activities associated with daily living in an independent manner).
Exclusion criteria
- Female
- Smoking
- Allergies to milk proteins
- Musculoskeletal disorders
- Use of any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications).
- Participation in any structured regular exercise program
- Chronic use of gastric acid suppressing medication or anti-coagulants
- Unstable weight over the last three months
- Pathologies of the gastrointestinal tract
- Blood donation in the 2 months prior to start of the study
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL52798.068.15 |
| OMON | NL-OMON29333 |