Effects of deoxycholic acid supplementation on insulin: role for the transmembrane G coupled protein receptor TGR5

Traditionally, bile acids (BAs) have been thought to serve the purpose of
emulsifying nutrients. However, evidence has
emerged from animal and in-vitro studies that BAs also play a role in the
regulation of energy metabolism, particularly
glucose and fat metabolism. This is mediated through the BA receptors TGR5 and
FXR. Modulating BA kinetics in mice has been shown to affect insulin
sensitivity

To investigate whether postprandial insulin release is affected by
administration of the secundary BA DCA

Healthy subjects will indergo a mixed meal test twice, once with and once
without oral administration of DCA. After the mixed meal, blood samples will be
taken at various timepoints.

Addition of deoxycholate to the standard meal (1x)

Burden:
2x clinic visits of 5 hours each
2x placement of IV cannula for blood sampling, 225 ml in total
1 oral administration of DCA (10 mg/kg)

Because of the time spent and the invasive measurements, we estimate the burden
of this study to be moderate

Risks:
low risk of hematoma and/or phlebitis (IV cannula)
low risk of diarrhoea and/or abdominal cramping (infrequently described after
prolonged use of DCA)
low risk of reversible AST increase (infrequently described after prolonged use
of DCA; reversible upon dscontinuation)

We estimate the risks to be low and the possible consequences to be negligible