The goal of this study is developing a non-invasive method to test for a specfic allergy using fingermarks.
ID
Source
Brief title
Condition
- Allergic conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Positive reaction after the skin prick test, whereby a drop of a solution
containing the allergen is placed on the skin, next to this a serie of needle
pricks allows the solution to enter the skin, resulting in a red, raised itchy
area (called a wheal). Positive staining after incubation of a specific
allergen in the fingermark.
Negative reaction after injection of a specific allergen after the skin prick
test. Negative staining after incuabation of a specific allergen in the
fingermark ( no staining).
Determination of specific allergies in as well the intracutaneous skin prick
test as the fingermark.
Saliva will be used as control --> Increased or no increased level of total
IgE
Secondary outcome
-
Background summary
Allergic reactions are caused by the natural defense system of the human body
that fights against harmless substances (allergens) present in the environment
(food, air etc). This reaction can cause symptoms, like a rash, running nose
and itchy eyes. To determine whether an individual is allergic to a specific
allergen, several tests are available, such as a skin prick test or blood
analyses. In most cases, these test are invasive and are recorded as painful to
the patient.
In this study a non-invasive method is tested. Instead of a skin-prick-test,
the allergens are incubated on fingermarks. It is known that antibodies
specific to an allergen are excreted in human sweat. Fingermarks are composed
of natural secretions, excreted via the pores to the surface of the skin. We
hypothesized that antibodies specific for an allergen are present in the
fingermark depositions.
Fingermarks are placed by the volunteers on a glass slide or nitrocellulose
membrane. The fingermarks are incubated with an allergen conjugated to a visual
enhancer. If the antibody specific to that particular allergen is present, a
positive staining will occur. Besides, placing the fingermarks, also a skin
prick test will be performed. The two test results will be compared, leading to
a conclusion, whether fingermarks can be used as non-invasive allergy test.
Saliva will be used as a controle, the presence of total IgE will be
investigated.
If fingermarks can be used as non-invasive method for allergy testing, it will
be of a major impact to the allergy diagnostics.
Study objective
The goal of this study is developing a non-invasive method to test for a
specfic allergy using fingermarks.
Study design
Observational study.
Study burden and risks
The nature of the burden is classified as minimal, considering that subjects
have to undergo the skin prick test and have to come to the Academic Medical
center for one session. The risks involved are negligible.
Meibergdreef 9
Amsterdam 1105 AZ
NL
Meibergdreef 9
Amsterdam 1105 AZ
NL
Listed location countries
Age
Inclusion criteria
- rhiniconjunctivitis +/- asthma
- allergic symptoms, provocation after contact with trees, grass, mites, dogs and/or cats.
- positieve skin pricktest for specific allergen in consistency with symptoms
-18 years and older.
- mentally competent
- no comorbidity which interferes with the skin prick test;control subject:
- no rhinoconjunctivitis, asthma of eczema
- 18 years or older
- mentally competent
- no comorbidity
- negative skinpricktest
Exclusion criteria
- younger than 18 years
- not mentally competent
- comorbidity, which interferes with the skinprick test
-negative skin prick test
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL44936.018.13 |