This study aims to examine the effects of timed protein supplementation on the endurance-based exercise training-induced changes in VO2max.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
Effect van duurtraining met eiwitsuppletie op spier en bloed
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
VO2max
Secondary outcome
10-km time trial
Muscle Fiber Adaptations
capillary density
gene expression
phosphorylation proteins
mitochondrial mass
Cytochrome synthesis activity
blood parameters
body Composition
waist circumference
body weight
BMI
Background summary
Regular endurance training improves oxygen uptake and transport, and ultimately
results in a better use of oxygen by the active muscles. Nutrition plays an
important role before, during and after exercise. Carbohydrates are best known
for the role they play in the rapid release of energy during exercise, protein
is mainly known for its role in muscle recovery after exercise. Previous
research has shown that protein supplementation optimizes the recovery of
strength training resulting in bigger en stronger muscles. For endurance
training, however, it is currently unclear whether protein supplementation does
improve adaptation over a longer period.
Study objective
This study aims to examine the effects of timed protein supplementation on the
endurance-based exercise training-induced changes in VO2max.
Study design
This study will be a double blind, randomized, placebo-controlled intervention
trial. The total study consists of a screening and an experimental part in
which we will focus on the effects of an exercise intervention program with
nutritional support on VO2max, in untrained healthy young men.
Intervention
Three times a week endurance training, for a total period of 12 weeks. The
exercise sessions in week 6 and 12 will be replaced by other exercise tests.
The training sessions last for 30 up to 60 minutes (alternated)
Group Intervention: protein supplement (30 g protein). After training and
before bedtime.
Placebo Group: carbohydrate supplement (30 g carbohydrate). After training and
before bedtime.
Study burden and risks
Maximal exercise testing: exercise testing can lead to muscle soreness. This is
harmless and disappear away after a few days.
Blood collection: despite the blood is taken with great care and is conducted
by an experienced nurse, it is possible that the puncture created a bruise.
These disappear after a few days.
Muscle biopsy: a muscle biopsy is a small procedure in which a small piece of
muscle is removed from the thigh. In total there will be 100-150 mg muscle
tissue be removed per muscle biopsy. The muscle biopsy is taken from the right
thigh. The skin and the underlying tissue are locally anesthetized. After this,
a small incision (0.5 cm) will be made in the skin and the muscle sheath.
Through this slice of a hollow needle is inserted with which a few chunks of
muscle to be disconnected. After taking the muscle biopsy the wound edges are
closed with a sterile bandage and a pressure bandage to minimize the chance of
bleeding. The incision will heal completely, but, in some cases, subjects keep
a little scar on the place of the biopsy. There could rise some local muscle
soreness the first two days after the muscle biopsy.
Stippeneng 4
Wageningen 6708 WE
NL
Stippeneng 4
Wageningen 6708 WE
NL
Listed location countries
Age
Inclusion criteria
* Male
* Age between 18 and 30 years of age
* BMI between 18.5 * 25 kg/m2
* Recreationally active, performing sports on a non-competitive basis
* Wmax * 5 *kg
* Willing to give muscle biopsies
* Willing to give blood samples
* Having a general-practitioner
* Consumption of alcohol beverages is less than 21 per week
* Able to perform three exercise sessions weekly for 12 weeks
Exclusion criteria
* Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, liver or renal disease, diabetes mellitus type 1 or 2)
* Having a lactose and/or gluten intolerance
* Use of systemic medication (with the exception of antihistaminic medication and the use of occasional painkillers)
* (Chronic) injuries of the locomotor system (e.g. musculoskeletal/orthopedic disorders) that can interfere with the intervention
* Participants who are enrolled in an interventional biomedical research project or have received an investigational new drug or product with the last 30 days prior to screening.
* Smokers and use of illicit drugs
* Blood donor during the study and in the three months before start of the study
* Employed, or intern, or working on a thesis at the department of Human Nutrition at Wageningen University & Research
* Participating in another scientific study (except EetMeetWeet)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL59885.081.16 |