In 15 patients of whom sputum will be collected (not in the other participating patients) :does depletion of eosinophils disturb the balance between Th2/Th1/Th17 cells in peripheral blooddoes depletion of eosinophils chance CD8 memory responses in…
ID
Source
Brief title
Condition
- Allergic conditions
- Bronchial disorders (excl neoplasms)
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
genotyping white blood cells
humoral parameters and cellular differentiation of mucosal tissue (sputum and
nasal lavage)
Secondary outcome
n.a.
Background summary
Eosinophilic granulocytes in murine studies display important immune-modulatory
properties on both the adaptive and innate immune response. Treatment of severe
asthma patients with anti-IL-5 (mepolizumab) will result in patients without
eosinophils. In this study we will examine the effect of depletion of
eosinophils on the adaptive and innate immune response. In addition we will
search for a biomarker that can serve as a predictor of efficacy.
Study objective
In 15 patients of whom sputum will be collected (not in the other participating
patients) :
does depletion of eosinophils disturb the balance between Th2/Th1/Th17 cells in
peripheral blood
does depletion of eosinophils chance CD8 memory responses in peripheral blood.
does depletion of eosinophils affect IgA and M1/M2 macrophage response at mucosa
In all patients we will search for a predictive biomarker.
Study design
Before mepolizumab treatment, one month after first dose and 9-12 months after
start blood will be drawn, a nasal lavage and a sputum will be induced.
Study burden and risks
per visit (3 times) blood will be drawn,a nasal lavage obtained and a sputum
induced. The burden and risk are being considered as minimal.
Meibergdreef 9 K0-150
Amsterdam 1105AZ
NL
Meibergdreef 9 K0-150
Amsterdam 1105AZ
NL
Listed location countries
Age
Inclusion criteria
severe asthma
Exclusion criteria
anemia, very low white blood cell count, anti-inflammatory and/or immunosuppressive medication other than used to treat asthma
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL56770.018.16 |