To study the impact of primary keratinocytes isolated of allergic contact dermatitis (ACD) cases on THP-1 cell activation.
ID
Source
Brief title
Condition
- Epidermal and dermal conditions
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Keratinocytes from the biopsies of allergic individuals and controls will be
isolated and propagated in vitro. They will be cocultured with the dendritic
cell surrogate THP-1 cells and exposed to purified terpene hydroperoxides. As
main study parameters/endpoints will be the release of inflammatory mediators
and expression of activation markers in and on both types.
Secondary outcome
N/A
Background summary
Rationale: The aim is to elucidate whether susceptibility factors arising from
diseased keratinocytes are implicated in the activation of the innate immune
response. In order to investigate this, keratinocytes from subjects with a
proven contact allergy (control: healthy subjects) will be harvested from a
skin biopsy.
Study objective
To study the impact of primary keratinocytes isolated of allergic contact
dermatitis (ACD) cases on THP-1 cell activation.
Study design
Laboratory study with keratinocytes, which will be isolated from biopsies from
both cases and healthy controls, and propagated in vitro in the lab (Trier
University) * laboratory study with an invasive procedure.
Intervention
Two skin biopsies will be taken from each case, one skin biopsy from each
healthy control. For cases, the biopsies will be taken from the skin area on
the volar side of both forearms, where on one arm a patch test will be
performed with either FM I or FM II, and on the other arm a patch test with
only petrolatum. The skin biopsies will be taken on day 3 after the patch tests
are applied. For controls, the skin biopsy will be taken from the volar side of
an forearm.
Study burden and risks
Duration of participation for cases will be 4 days. A patch test containing
either FM I or FM II, depending on their sensitivity, will be applied on the
volar aspect of one forearm, with a patch test containing only petrolatum on
the other arm, both for 48 hours. At 72 hours the skin biopsies will be takes,
one from the elicitation reaction from the FM I/II patch test and one from the
petrolatum occluded skin. Each volunteer will have to visit the UMCG two times,
once to apply the patch test, and a second time to take the skin biopsies.
For healthy controls, there is one visit to the UMCG in which a biopsy will be
taken of healthy skin from the forearm.
Hanzeplein 1
Groningen 9713GZ
NL
Hanzeplein 1
Groningen 9713GZ
NL
Listed location countries
Age
Inclusion criteria
Case group:
- A proven contact allergy (by previous patch testing at the department of dermatology in the UMCG) to either/both FM I and/or FM II.
- Caucasian female
- Age between 20 and 50 years
- Legal competence;Control group:
- Caucasian female
- Age between 20 and 50 years
- No clinical history of dermatitis reaction to fragranced products
- Legal competence
Exclusion criteria
- Skin-anomalies on the forearm such as active eczema
- Current atopic dermatitis or psoriasis
- The use of immunosuppressive medication during the study or in the four weeks
before inclusion (oral corticosteroids, cyclosporine, methotrexate, azathioprine and
biologicals)
- Legally incompetent
- Difficulty understanding spoken and written information in Dutch.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL65736.042.18 |