To assess whether whey protein supplementation during the days before and after muscle-damaging eccentric exercise improves recovery of muscle strength, compared to placebo?Secondary objective(s) are: To assess whether whey protein supplementation…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
spierschade na fysieke inspanning
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Change in muscle strength of the quadriceps muscles (isokinetic Maximal
Voluntary Contraction (MVC)) up to 3 days after eccentric exercise, compared to
pre-exercise strength.
Secondary outcome
* Isometric MVC
* Jump height assessed by vertical countermovement jump.
* Thigh circumference.
* Delayed onset muscle soreness (DOMS) assessed by VAS (100 mm)
* Muscle soreness assessed by a 7 point Likert-scale for retrospective pain.
* Muscle tenderness assessed by a manual muscle myometer.
* Rated perceived exertion (RPE), assessed by the BORG scale.
* Plasma creatine kinase (CK) concentrations
* Plasma intestinal fatty acid binding protein (I-FABP) concentrations
* Circulating neutrophils and monocytes (white blood cell count) in whole blood
* Plasma concentrations of IL-6, MCP-1, IL-1ra, IL-8 and IL-10
* Plasma concentrations of NGF
Background summary
Delayed onset muscle soreness (DOMS) develops 24-48 hours after strenuous
exercise biased toward eccentric (muscle lengthening) muscle actions. Soreness
is accompanied by a prolonged strength loss, a reduced range of motion, and
elevated levels of muscle damage markers in the blood. Reported study results
are not consistent with respect to the acute benefits of protein
supplementation on reductions in muscle damage and enhanced recovery of muscle
function. We hypothesize that supplementation with whey protein is efficacious
in improving recovery following eccentric exercise when given in a daily dose
of 60 gram in the days before and after the exercise. Reduction of muscle
soreness is likely to stimulate people to remain active, and thereby to improve
their physical condition and support healthy aging.
Study objective
To assess whether whey protein supplementation during the days before and after
muscle-damaging eccentric exercise improves recovery of muscle strength,
compared to placebo?
Secondary objective(s) are:
To assess whether whey protein supplementation before and after muscle-damaging
eccentric exercise reduces
* muscle soreness?
* blood markers of muscle damage?
* blood markers of inflammation and immune response?
Study design
Double-blind placebo controlled cross-over intervention study.
Intervention
Volunteers will consume either a whey protein supplement (60 g protein/d) or an
isocaloric placebo supplement (maltodextrin), additional to their habitual
diet, during 9 days. A dose of 20 g protein will be consumed in the morning,
between breakfast and lunch, and a dose of 40 g protein will be consumed in the
evening.
Study burden and risks
Participants will visit the exercise lab 6 times. Overall, 9 blood samples will
be drawn (of which 6 at one visit, via a canula). Participants will have to
perform an exhaustive jump test, which is meant to induce moderate muscle
damage and muscle soreness. Muscle function tests will be done 6 times (of
which 3 at one visit). Volunteers will have to complete an online 24h diet
recall over 4 days, and adhere to dietary restrictions and wear an
accelerometer during the 9-day intervention period. Test products are
commercially available, food-grade food ingredients that are safe in the
amounts consumed in this study.
Kernhemseweg 2
Ede 6718ZB
NL
Kernhemseweg 2
Ede 6718ZB
NL
Listed location countries
Age
Inclusion criteria
* Male
* Age *18 and *35 years.
* BMI *18.5 and *27.5 kg/m2
* Healthy as assessed by the NIZO lifestyle and health questionnaire.
* Recreationally active, defined as performing physical activities on a recreational basis for a maximal duration of 5 hours per week.
* Veins suitable for cannulation (blood sampling)
* Having given written informed consent
Exclusion criteria
* Having a history of medical or surgical events that may significantly affect the study outcome, to be decided by the principal investigator
* Participating in resistance exercise or exercise with a major eccentric component (e.g. (trail) running, football, volleyball, basketball, track and field) in the last 6 months.
* Regular use of the following medication: corticosteroids, antihistamines, NSAID
* Smoking
* Regular use of protein supplements
* Mental status that is incompatible with the proper conduct of the study
* A self-reported reported lactose intolerance, allergy or sensitivity to dairy ingredients
* Evidence of current excessive alcohol consumption (>21 units/week) or drug (ab)use, and not willing/able to stop this during the study.
* Reported slimming or medically prescribed diet
* Participation in any clinical trial including blood sampling and/or administration of substances up to 30 days before Day 01 of this study
* Recent blood donation (<1 month prior to Day 01 of the study)
* Personnel of HAN, NIZO or FrieslandCampina, their partner and their first and second degree relatives
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL68027.072.18 |
| OMON | NL-OMON27140 |