Assessing different modalities of ektacytometry in diagnosing thalassemia and/or iron disorders. Investigating the influence of hemoglobinopathies and iron disorders on red cell membrane deformability in comoparison with healthy controls.
ID
Source
Brief title
Condition
- Haemoglobinopathies
- Blood and lymphatic system disorders congenital
- Iron and trace metal metabolism disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Investigating ektacytometry in 6 different groups of patients
(alpha-thalassemia trait, alpha-thalassemia homozygote, haemoglobin H disease
and other more rare variants, beta-thalassemia intermedia, iron deficiency
anemia and hereditary hemochromatosis).
Secondary outcome
Correlating ektacytometry data of these patients to clinical data, morphology
of RBCs, haematological parameters and iron status, oxidative stress and
nitrosylation assays, pyruvate kinase activity and stability, methemoglobin and
oxygen saturation.
Background summary
Ektacytometry is a method which can be used to investigate red blood cell
deformability under several different circumstances. Laser Optical Rotational
Cell Analyser (Lorca) is a next generation ektacytometer which can be used to
measure different aspects of red blood cell (RBC) deformability.
Alpha- and beta-thalassemias are hemoglobinopathies in which a defect in alpha
or beta globin chains causes red blood cell changes and eventually haemolytic
anemia. Iron deficiency causes microcytosis and hypochromic red blood cells.
Iron overload in hemochromatosis causes oxidative stress which results in
damage to red blood cells. The damage on red blood cells caused by these
different pathologies have different effects on their deformability. This study
investigates the deformability of different hemoglobinopathies and iron
disorders on red blood cell deformability measured with the Laser optical
rotational cell analyser (Lorca) a next generation ektacyometer.
Study objective
Assessing different modalities of ektacytometry in diagnosing thalassemia
and/or iron disorders. Investigating the influence of hemoglobinopathies and
iron disorders on red cell membrane deformability in comoparison with healthy
controls.
Study design
cross-sectional observational study
Study burden and risks
The study will investigate blood of patients. If possible the venipuncture will
be combined with a routine visit. Otherwise one venipuncture is needed.
The subjects will not directly benefit from this study.
Heidelberglaan 100
Utrecht 3508 GA
NL
Heidelberglaan 100
Utrecht 3508 GA
NL
Listed location countries
Age
Inclusion criteria
* > 18 years
* No regular blood transfusions
* Have alpha-thalassemia trait, homozygote alpha thalassemia, Hemoglobin H or other more rare variants where 3 alleles are affected, beta-thalassemia intermedia, iron deficiency anemia or hereditary hemochromatosis
* Be able to give informed consent
Exclusion criteria
* Suffering from another serious condition
* Fever at time of venepuncture
* Inflammatory condition at time of venepuncture
* Not able to give informed consent
* Regular blood transfusions
* Intra venous iron therapy in past 6 months
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL56731.041.16 |
| Other | volgt |
| OMON | NL-OMON22102 |