The effects of quark ingestion with or without prior exercise on muscle protein synthesis rates in young and old men.
ID
Source
Brief title
Condition
- Muscle disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Increase in postprandial muscle protein synthesis in rest and after exercise
compared to rested postabsorptive muscle protein synthesis
Secondary outcome
whole body protein metabolism
plasma amino acids
plasma glucose and insulin.
Background summary
Muscles are built up out of proteins. These protein consists of little building
blocks: amino acids. By consuming sufficient amino acids/protein in our
nutrition, we make sure our body has enough building blocks to maintain or
increase our muscle mass. To maintain or increase muscle mass with aging, it is
advised to combine exercise with sufficient protein intake. A high quality
source of protein is milk. However, it is important to get more insight in the
anabolic properties of milk derived protein, like quark.
Study objective
The effects of quark ingestion with or without prior exercise on muscle protein
synthesis rates in young and old men.
Study design
A single-intervention, within-subjects experimental trial with 2 different
groups (young and old).
Intervention
300g quark
resistance exercise
Study burden and risks
The burden and risks associated with participation are small. Insertion of the
catheters and the venapunction are comparable to a blood draw and could result
in a small hematoma. Muscle biopsies will be taken under local anesthesia by an
experienced physician, but may cause some minor discomfort for maximally up to
24 h after completion. The discomfort is comparable to muscle soreness or the
pain one has after bumping into a table. We will take 12 blood samples (10 mL)
of which 1 during the screening and 11 during the experimental trial. The total
amount of blood we draw is less than half the amount of a blood donation and
will be completely restored in approximately 1 month. Participants come to the
university twice: 1 screening (3 hours) and 1 experimental trial (entire day).
For both the screening and the experimental trial, participants have to be
fasted, so they are not allowed to eat and drink (except for water) from 22h00
the evening before. Also, 3 days prior to the experimental trial participants
should keep their diet as constant as possible, do not perform any type of
intense physical exercise, and do not consume alcohol. During the screening we
will perform a DEXA and a strength test. Furthermore, we will ask the
participants to fill out a medical questionnaire and record their food intake
and activity for 2 days prior to the experimental trial. During the
experimental trial, we will collect muscle and blood samples, and participants
have to perform resistance exercise and consume 300g quark. There is no direct
benefit for the participants, only their contribution to scientific knowledge.
Universiteitssingel 50
Maastricht 6229 ER
NL
Universiteitssingel 50
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
* Healthy males
* Age between 18 and 35 y or 65 and 85 y inclusive
* BMI between 18.5 and 30 kg/m2
Exclusion criteria
* Allergies to milk proteins
* Lactose intolerant
* Smoking
* Phenylketonuria
* Diabetes Mellitus (diagnosed, or fasting glucose >7.0 mmol/L, or HbA1c >6.5)
* Diagnosed GI tract disorders or diseases
* Arthritic conditions
* A history of neuromuscular problems
* Any medications known to affect protein metabolism (i.e. corticosteroids,
non-steroidal anti-inflammatories, or prescription strength acne medications).
* Use of certain anticoagulants (use of thrombocyte aggregation inhibitors such
as acetylsalicylic acid and carbasalaatcalcium is permitted. Use of other
thrombocyte aggregation inhibitors will be discussed with the responsible
physician)
* Blood donation within 2 months of study initiation
* Hypertension (according to WHO criteria; >90/140 mmHg)
* Recent participation in amino acid tracer studies (less than 1 year ago)
* Physical activity (not training more than 3 times per week and no structured
resistance training.)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL71731.068.19 |