The aim of the project is to validate biomarkers for the development of a peripheral blood test which can distinguish early inflammation between various forms of IBD, with the use of novel techniques, such as mass cytometry and RNAseq.
ID
Source
Brief title
Condition
- Gastrointestinal inflammatory conditions
- Autoimmune disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Definition of final staining panel which can allow for early inflammation
dectection as well as distinguish between various forms of IBD , using
peripheral blood samples.
Secondary outcome
1. Whether with this peripheral blood test a flare can be predicted
2. Whether this peripheral blood test can distinguish CD from UC patients.
3. To see how the medication of IBD patients can affect immune cell activation
markers within peripheral blood.
Background summary
Innate lymphoid cells (ILCs) are important regulators of epithelial tissue
integrity during homeostasis. We have recently focused on innate lymphoid
cells (ILCs) in peripheral blood. In peripheral blood of healthy individuals
ILCs exhibit a resting phenotype, expressing the lymph node homing receptor
CD62L, suggesting that ILCs travel through lymph nodes just like naïve T cells
do. However, ILCs in peripheral blood of Crohn*s patients exhibited a strongly
reduced CD62L expression. Our results led us to hypothesize that ILCs will
become activated in lymph nodes that drain an inflammatory site, upon which
they will lose their CD62L expression. In addition, the gut homing molecule
α4β7 was expressed on ILC present in peripheral blood of Crohn*s patients and
mutually exclusive with CD62L expression, allowing their migration to the
intestine. In addition, we did not observe such a consistent reduction of CD62L
expression on PB ILC in patients suffering from ulcerative colitis. We
hypothesize that phenotypic and functional changes in peripheral blood innate
lymphoid cells will allow for early detection of disease (re)activation in IBD
patients as well as distinction between various forms of IBD.
Study objective
The aim of the project is to validate biomarkers for the development of a
peripheral blood test which can distinguish early inflammation between various
forms of IBD, with the use of novel techniques, such as mass cytometry and
RNAseq.
Study design
The proposed study is designed as an prospective observational study in the
setting of the outpatient clinic of the VU medical center.
Study burden and risks
Inflammatory bowel disease has been diagnosed in 9 out of 1000 citizens in the
Netherlands. It is a unpleasant conditions which results in diarrhoea, pain in
the abdominal region and discomfort. For this study, liquid biopsies are
needed. The low risks for individual participating patients are insignificant
to the knowledge that can be provided by this study. The project is aimed at
improving the early diagnosis of IBD patients. By the combination of two novel
techniques, much information can be obtained from very limited amount of
peripheral blood. By analysing enough patients the obtained data will allow a
better insight in the different subgroups within all Crohn*s and Ulcerative
colitis patients. Improving the diagnosis of IBD patients will ultimately lead
to better treatment and care for Crohn*s patients, which will reduce the burden
on family as well as on society.
De Boelelaan 1108
Amsterdam 1081 HZ
NL
De Boelelaan 1108
Amsterdam 1081 HZ
NL
Listed location countries
Age
Inclusion criteria
Patients:
- Provide informed consent
- Within age 18 until 75
- Diagnosed with Crohn*s disease or ulcerative Colitis
Healthy volunteers:
- Provide informed consent
- Within age 18 until 75
- Healthy, without a medical history of any inflammatory disease
Exclusion criteria
-Patients:
- Younger than 18 and older than 75
- Patients who cannot be properly informed about the risks due to an
insufficient ability to speak Dutch or English
- History of another inflammatory diseaseHealthy volunteer:
- History of any inflammatory disease
- Younger than 18 and older than 75
- Employees of the department of Gastroenterology
- Family or friends of employees of the department of Gastroenterology
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL65801.029.18 |