Primary Objective: * To compare the expression of Th1 and Th2 from Europeans with those of Africans living in urban, semi urban and rural areas.Secondary Objective: * To explore the functional phenotypes of Th1 and Th2 using mass cytometry cells as…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
Immunological profile; enviromental exposure
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Primary endpoint
* The percentages of interleukin (IL-) 4, IL-5, IL13 cytokine producing CD4+ T
cells as indicator of Th2 response and interferon gamma (IFNg) producing CD4+ T
cells as Th1 cells, in PBMC from Europeans with those of Africans living in
urban, semi urban and rural areas
Secondary outcome
Secondary endpoint
* Characterization of PBMCs in terms of lineages of T cells, B cells, monocytes
and dendritic cells as well as innate lymphocytes and their subsets and
clusters in Europeans in addition to Africans living in urban, semi urban and
rural areas.
Exploratory endpoints
* Data set on transcriptome of cells identified to pay a determining role in
European, African rural, semi urban and rural profiles.
* Combined analysis of the high dimensional data obtained by mass cytometry and
RNA sequencing
* Establishment of SOPs to be used at the Cheikh Anta Diop University in
Senegal for further investigation of vaccine responses
Background summary
As the immune system is central to the success of vaccines and because of the
recent indications that strong geographical differences may define
immunological footprints, assessment of the environmental impact on the immune
system need to form an integral part of any serious attempt for vaccine
development. Indeed, in studies comparing immune responses of rural and urban
Africans as well as matched Europeans, we have shown that there are major
differences that cannot be accounted for by genetic variation only .
Study objective
Primary Objective:
* To compare the expression of Th1 and Th2 from Europeans with those of
Africans living in urban, semi urban and rural areas.
Secondary Objective:
* To explore the functional phenotypes of Th1 and Th2 using mass cytometry
cells as well as their transcriptional signature following environmental
settings
* To analyse the high dimensional data obtained by mass cytometry and RNA
sequencing
* Training in complex immunological analysis of samples
Exploratory Objective:
* To explore the transcriptional signature of cells following environmental
settings
* To analyse the high dimensional data obtained by mass cytometry and RNA
sequencing
* Training in complex immunological analysis of samples
Study design
This is a prospective cohort study involving rural, semi-urban, and urban
populations with long term differential environmental exposures that are
suspected to impact the development of the immune system. We will gather data
on the exposure period to each environmental setting from individual
questionnaires at time of inclusion. Sampling will only be performed at
baseline through a single venipuncture procedure.
Study burden and risks
For the whole study, participants will only need the clinic once for
venipuncture and filling out the questionnaires. The only physical
interventions in this study involves collection of venous blood that may be
associated with a slight pain and discomfort due to the middle penetration but
those regress within minutes. There are no direct benefits for the volunteers
Avenue Cheikh Anta Diop, Fann 5005
Dakar 5005
SN
Avenue Cheikh Anta Diop, Fann 5005
Dakar 5005
SN
Listed location countries
Age
Inclusion criteria
In order to be eligible to participate in this study, a subject must meet all of the following criteria:
- Living in the urban, semi-urban or rural setting preferably lifelong (not less than 10 years)
- Be 18-40 years of age
Exclusion criteria
A potential subject who meets any of the following criteria will be excluded from participation in this study:;- Any history of diabetes, arterial hypertension, chronic renal failure
- Signs or history of infection at the time of inclusion
- Any history or clinical signs of inflammatory diseases
Design
Recruitment
metc-ldd@lumc.nl
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In other registers
| Register | ID |
|---|---|
| CCMO | NL66287.058.18 |