This study aims to further investigate neutrophil viability as marker for neutrophil cell dead in patients with a decreased leukocyte viability factor and investigate its impact on neutrophil functioning.
ID
Source
Brief title
Condition
- Immune disorders NEC
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The primary endpoint is neutrophil cell death, determined by double staining of
Annexin V and PI. This will be compared to the decreased viability factor of
the initial routine blood sample to validate the viability factor.
Secondary outcome
Secondary endpoints are neutrophil phagocytosis and responsiveness to fMLP.
Background summary
Previous studies showed a decreased neutrophil viability in critically ill
patients. Neutrophil viability was measured using propidium iodide (PI)
staining, which visualizes diminished membrane integrity and thus cell necrosis
or apoptosis. Not much is known about a decreased neutrophil viability and its
implications for neutrophil functioning.
Study objective
This study aims to further investigate neutrophil viability as marker for
neutrophil cell dead in patients with a decreased leukocyte viability factor
and investigate its impact on neutrophil functioning.
Study design
prospective cohort series, diagnostic
Study burden and risks
A total of two 4 milliliter blood containers will be collected from the
patients. Sampling this amount of blood will have a negligible influence on
total blood volume. In total, one venipuncture is required to obtain the blood
samples and if the patient has a central venous or peripheral arterial catheter
in situ, no punctures are required.
Heidelberglaan 100
Utrecht 3584 CX
NL
Heidelberglaan 100
Utrecht 3584 CX
NL
Listed location countries
Age
Inclusion criteria
leukocyte viability factor * 0.95
Exclusion criteria
Immunosuppressive drugs (e.g. chemotoxic and cytostatic drugs) or positive HIV-status or use of clozapine medication
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL60543.041.17 |