Primary: To investigate the effect of protein density and texture on gastric emptying of protein-rich solid food. Secondary: To investigate the use of MRI T2 and T1 of gastric content on indicating protein hydrolysis in stomach.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
Protein digestibility
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Gastric emptying half-time (GE-t50) measured by MRI.
Secondary outcome
Protein hydrolyses will be quantified by MRI T1 and T2 during digestion.
Background summary
Gastric digestion is crucial to food structure breakdown, and subsequent
digestion and nutrient absorption. Several studies have shown that protein-rich
structured food (i.e. whey protein gel) with higher hardness digests slower
during in vitro gastric digestion. However, no in vivo human data are
available. In addition, a recent in vitro study showed that the hydrolysis of
protein could be monitored by transverse relaxation time (T2), however the
feasibility of MRI parameters (T1 and T2) still needs to be investigated in
vivo.
Study objective
Primary: To investigate the effect of protein density and texture on gastric
emptying of protein-rich solid food.
Secondary: To investigate the use of MRI T2 and T1 of gastric content on
indicating protein hydrolysis in stomach.
Study design
Three treatments will be compared in a randomized crossover design.
Intervention
Participants consume three types of whey protein gels (200 g): 1) low protein
density + low hardness, 2) low protein density + high hardness, 3) high protein
density + high hardness. MRI abdominal scans, and appetite and wellbeing
ratings will be obtained before ingestion and every 10 min until 90 min after
ingestion. After the MRI session, satiety will be measured by an ad libitum
meal. MRI scans will be used to calculate gastric emptying half-time and MRI T1
and T2 will be used to quantify hydrolysis of protein.
Study burden and risks
The risks associated with participation are negligible. MRI is a safe and
commonly used medical imaging modality. The stimuli consist of normally
consumed food ingredients. The burden associated with participation consists of
three 2 hours visits, which require an overnight fast and lying in the MRI
scanner for 80 min (90 min - consumption time). This may cause minor
discomfort. The study is non-therapeutic to the participants.
Stippeneng 4
Wageningen 6708 WE
NL
Stippeneng 4
Wageningen 6708 WE
NL
Listed location countries
Age
Inclusion criteria
18-45 years old
Male
Apparently healthy (self-reported)
Healthy body weight, BMI 18.5 - 25 kg/m2
Speak and understand English without difficulty
Exclusion criteria
* Vegan
* Whey protein allergy or intolerance (self-reported)
* Food sensitivities towards any of the ingredients
* Gastric disorders or regular gastric complaints, such as heart burn
* Use of proton pump inhibitors or other medication which alters the normal
functioning of the stomach
* Having a contra-indication to MRI scanning (including, but not limited to):
* Pacemakers and defibrillators
* Intraorbital or intraocular metallic fragments
* Ferromagnetic implants
* Claustrofobia
* An employ from Human Nutrition and Health (WUR)
* A student or intern from Chair of Sensory Science and Eating Behaviour (WUR)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL74166.081.20 |
| Other | We have submitted the trail in Netherlands Trial Register. The identification number will be informed. |
| OMON | NL-OMON20045 |