To compare the post-prandial muscle protein synthetic response following ingestion of a whole-food meal (560kCal; 30g protein total) before and after a 8-week passive heat treatment intervention in healthy older men and women.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
anabole resistentie
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Fractional synthetic rate (FSR) of muscle protein synthesis (MPS) over the 4
hour period following meal ingestion
Secondary outcome
Secondary endpoint is the skeletal muscle microvascular perfusion capacity in
rest and 1h following meal ingestion assessed by contrast enhanced ultrasound.
change in post-prandial plasma amino acid, glucose and insulin concentration.
Changes in type I and type II muscle fiber capillarization assessed in the
post-absorptive muscle biopsy samples by the use of immunohistochemistry
Background summary
Aging is characterized by loss in skeletal muscle mass, resulting in reduced
health and physical performance in daily life of older adults. Dietary protein
intake and sufficient physical activity are two key factors in the maintenance
of skeletal muscle tissue. During aging, however, people lose the sensitivity
to respond to these signals. This loss is referred to as anabolic resistance.
Skeletal muscle perfusion plays an essential role in the delivery of oxygen,
signals and nutrients required for muscle tissue maintenance. The age-related
reduction in muscle perfusion capacity is suggested to be a potential cause in
the development of anabolic resistance. Today, exercise training is considered
the only effective intervention strategy to improve skeletal muscle perfusion
capacity and thereby counteract anabolic resistance in older adults. Though
this intervention strategy is effective, a large group of elderly struggle to
comply with intense exercise training programs. In this research study we will
assess whether passive heat treatment in the form of sauna bathing can improve
muscle tissue perfusion capacity and reduce anabolic resistance to food intake
in order to combat the long-term age-related lose in skeletal muscle mass.
Study objective
To compare the post-prandial muscle protein synthetic response following
ingestion of a whole-food meal (560kCal; 30g protein total) before and after a
8-week passive heat treatment intervention in healthy older men and women.
Study design
Pre - post within subject design
Intervention
A 8-week passive heat treatment (PHT) intervention at the department of Human
Biology, Maastricht University. The PHT intervention will consist of 3 times
per week (~45min per session) sitting in a infrared sauna cabin (HM-LSE-3
Professional edition, Health Mate, Belgium).
Study burden and risks
The burden and risks involved in participating in this experiment are small. A
DEXA scan will be done to assess body composition where the level of radiation
is very low compared to the background radiation level in the Netherlands.
The participants will participate in *whole-meal test days *(one before and one
after PHT) of ~9h. Insertion of the catheters during the test days is
comparable to a normal blood draw and the only risk is a small local hematoma.
During each of the 2 experimental test days 16 blood samples (170mL/day) will
be obtained. The total amount of blood collected during this study is less than
the amount of a blood donation and will be completely restored in approximately
1 month. The stable isotope amino acids tracers that will be infused
intravenously during the experimental trial are produced according to GMP
standards and are safe for human use. Throughout each whole-meal test day,
three muscle biopsies will be obtained under local anesthesia by an experienced
physician, but may cause some minor discomfort. The discomfort is comparable to
muscle soreness or the pain one has after bumping into the corner of a table.
For each whole-meal test day visit participants are required to come to the
university in a fasted state, not having consumed any food or beverages (except
for water) as from 22:00 the evening before. Also, 2 days prior to the
experimental test days participants need to record their food intake and
activities performed. During these 2 days participants are not allowed to
perform heavy physical exercise or drink alcohol.
A commercially available infrared sauna will be used for the PHT-intervention.
Participants will perform 3 PHT session per week for 8 weeks at the university,
each session will last 30-40 minutes. For healthy older adults there is very
little risk of being exposed to this PHT-protocol. Subjects will be provided a
rehydration protocol to replace the fluid that was lost during each PHT
session.
universiteitssingel 50
Maastricht 6200 MD
NL
universiteitssingel 50
Maastricht 6200 MD
NL
Listed location countries
Age
Inclusion criteria
Healthy volunteers between the age 65 and 85 years
Exclusion criteria
• Allergy for one of the food items used
• >5% weight change in the previous 6 months
• Participating in a structured (progressive) exercise program
• Frequent (more than once per week) user of infrared (or traditional) sauna in
the past 3 months
• Smoking
• Diagnosed cardiovasular disease
• Diagnosed musculoskeletal, GI tract, metabolic (e.g. diabetes) or pulmonary
(e.g. COPD) disorders
• Use of any medications known to affect protein metabolism (i.e.
corticosteroids, non-steroidal anti-inflammatories).
• Chronic use of gastric acid suppressing medication and/ or anti-coagulants
• Recent (<1 year) participation in amino acid tracer
(L-[ring-13C6]-phenylalanine and L-[3,5-2H2]-tyrosine) studies
• Blood donation in the past 2 months
• Strict vegetarian diet
• Known allergic reaction to ultrasound contrast agent
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL79421.068.21 |
| Other | zal worden geregistreerd na METC goedkeuring |