To assess the impact of protein in a solid (bar) and liquid (drink) form on post-prandial amino acid responses in vivo in healthy young women.
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
Eiwit vertering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
To compare the impact of protein in a solid (bar) and liquid (drink) form on
post-prandial plasma amino acid concentration in vivo in healthy young females.
Secondary outcome
To compare the impact of protein in a solid (bar) and liquid (drink) form on
peak post-prandial amino acid concentration, overall post-prandial glucose and
insulin responses, and hunger, desire to eat, and fullness in vivo in healthy
young females.
Background summary
Protein intake is an essential stimulus for protein anabolism. Protein
anabolism is modulated by plasma amino acid availability. Various aspects of
the dietary protein matrix have been shown to impact the post-prandial amino
acid response. However, it has not yet been investigated if ingestion of
protein in a solid (bar) form results in different post-prandial amino acid
responses when compared to a liquid (drink) form.
Study objective
To assess the impact of protein in a solid (bar) and liquid (drink) form on
post-prandial amino acid responses in vivo in healthy young women.
Study design
Randomized, cross-over study design.
Intervention
All subjects will perform two experiments in randomized order during which they
will ingest 20 g of milk protein as a drink or a bar. After ingestion, blood
samples will be taken at regular intervals during a 4 hour period.
Study burden and risks
The burden and risks with participation are small. During the screening, a DEXA
scan will be done to assess body composition, where the level of radiation is
very low compared to the background radiation level in the Netherlands.
Furthermore, we will ask the participants to fill out a medical questionnaire.
Insertion of the catheter during the test days is comparable to a blood draw
and could result in a small hematoma. We will take 11 blood samples during the
experimental period. The total amount of blood we draw (110 mL) is much less
than the amount of a blood donation (500 mL) and will be completely restored in
approximately 1 month. Participants will come to the university three times: 1
screening (~1h) and 2 experimental days (~4.5h). On the experimental test day,
the subjects will be asked to remain fasted (with the exception of the
experimental drink or bar). In addition, subjects will be asked in the two days
prior to the test day not to perform any type of intense physical activity and
to avoid consuming caffeine and alcohol in the 12h and 24h prior to the test
day, respectively. Participants will be asked to record their nutritional
intake and daily activities in the two days prior to the experimental test day.
There is no direct benefit to the participant, only their contribution to the
scientific knowledge on the impact of the dietary protein matrix on
post-prandial plasma amino acid responses.
Universiteitssingel 50
Maastricht 6229 ER
NL
Universiteitssingel 50
Maastricht 6229 ER
NL
Listed location countries
Age
Inclusion criteria
- Females
- Aged between 18-35 years
- Healthy, recreationally active (exercise at least 1 per two weeks and maximum
4 days a week)
- 18.5 <= BMI <= 30 kg/m2
- No physical limitations (i.e. able to perform all activities associated with
daily living in an independent manner).
Exclusion criteria
- Smoking
- Lactose intolerant or allergies to milk proteins
- Musculoskeletal disorders
- Metabolic disorders
- Use of any medications known to affect protein metabolism (i.e.
corticosteroids, non-steroidal anti-inflammatories, or prescribed acne
medications).
- Chronic use of gastric acid suppressing medication or anti-coagulants
- Unstable weight over the last three months
- Diagnosed GI tract disorders or diseases
- Blood donation in the past 2 months
- Pregnant
- Third generation oral contraceptives
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
In other registers
| Register | ID |
|---|---|
| CCMO | NL77723.068.21 |
| OMON | NL-OMON24741 |