In the current study we aim to measure the postprandial amino acid uptake kinetics of barley rice protein, and compare this to pea protein. A benchmark protein (whey) will be included for comparability of amino acid uptake of different protein…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
opname van aminozuren
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The main study parameters are total amino acid (AA) and essential amino acid
(EAA) uptake kinetics e.g. the appearance of free amino acids in blood samples
collected before and after the postprandial protein load.
Secondary outcome
Plasma glucose and insulin levels will be determined before and after
postprandial protein load
Background summary
Barley rice protein is extracted from brewers spent grain (BSG), the most
voluminous by-product of the beer brewing industry. Valorization and
utilization of spent grain protein is of great interest in terms of health and
sustainability. However, the knowledge of uptake kinetics of this protein
concentrate is insufficient at this point.
Study objective
In the current study we aim to measure the postprandial amino acid uptake
kinetics of barley rice protein, and compare this to pea protein. A benchmark
protein (whey) will be included for comparability of amino acid uptake of
different protein sources.
Study design
The study is a randomized, cross-over, double blind, controlled trial.
Intervention
During each visit, research subjects will receive one of the three protein
concentrates (barley rice protein, pea protein or whey protein) dissolved in
water, representing a 20g protein load, in randomized order.
Study burden and risks
In this study we will include healthy research subjects based on the study
criteria and a health questionnaire. There are minor risks for the research
subjects of this study. There are no direct benefits for the research subjects.
The total amount of blood collected (360 ml) is spread over three weeks and we
will exclude subjects with anaemia. Blood collection will therefore not be
expected to cause any problems. Research subjects that will participate in the
study will invest approximately 20 hours during the trial.
Bornse Weilanden 9
Wageningen 6708 WG
NL
Bornse Weilanden 9
Wageningen 6708 WG
NL
Listed location countries
Age
Inclusion criteria
* Apparently healthy men and women;
* Age between 18 and 40 years;
* Body mass index (BMI) between 18.5 and 30 kg/m2 ;
* Having veins suitable for blood sampling via a catheter (judged by study
nurse/ medical doctor).
Exclusion criteria
* Any metabolic, gastrointestinal, inflammatory or chronic disease (such as
diabetes, anaemia, hepatitis, cardiovascular disease),or having a condition or
disease that may lead to an impaired immune system;
* History of gastrointestinal surgery or having (serious) gastrointestinal
complaints;
* History of liver dysfunction (cirrhosis, hepatitis) or liver surgery;
* Kidney dysfunction (self-reported);
* Any use of medication that may suppress the immune system, this will be
judged by the medical supervisor;
* Use of medication that may influence the study results, such as gastric acid
inhibitors, laxatives, stomach protectors and drugs that can affect intestinal
motility, this will be judged by the medical supervisor;
* Anaemia (Hb values <7.5 mmol/L for women and <8.5 mmol/L for men);
* Reported slimming, medically prescribed or other extreme diets;
* Use of protein supplements;
* Not willing to give up blood donation during the study;
* Current smokers;
* Alcohol intake *4 glasses of alcoholic beverages per day;
* Pregnant, lactating or wishing to become pregnant in the period of the study
(self-reported);
* Abuse of hard drugs;
* Having food allergies and/or intolerances (e.g. for gluten);
* Not having a general practitioner;
* Participation in another clinical trial at the same time;
* Being an employee of the department Food, Health & Consumer Research of
Wageningen Food & Biobased Research.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| Other | het traject loopt |
| CCMO | NL80654.091.22 |