The objective of this study is to investigate the feasibility of using MT and CEST MRI for in vivo monitoring of gastric protein digestion (coagulation and breakdown). This will be done by studying the effect of heating on gastric protein…
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
fysiologie van eiwit vertering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
1. Average MTR of the stomach content over time (a measure of semi-solid
protein content)
Secondary outcome
1. Average CESTR of the stomach content over time (a measure of soluble
protein/peptide content)
Background summary
Gastric digestion is the first step in the breakdown of dietary proteins, and
is therefore important for further breakdown in the intestines and subsequent
absorption of amino acids. Food processing, such as heating can modify the
structure and digestibility of proteins. Digestion of dietary protein, and how
this is affected by heating is often studied using in vitro digestion models
that mimic the digestive tract of humans. However, outcomes from these models
need to be verified using in vivo digestion data from humans. Such data can in
turn be used to improve digestion models. Magnetic resonance imaging (MRI) may
potentially be used to non-invasively monitor both in vivo and in vitro protein
digestion, and hence, may bridge the gap between in vitro digestion models and
real-life digestion physiology. Here we propose a feasibility study, in which
we will evaluate whether MT and CEST MRI can be used for monitoring protein
digestion in vivo in humans. Pasteurized and extensively heated skim milk (80
oC, 30 min) will be used as a test case to demonstrate the ability of these MRI
methods to detect relevant differences in (protein) digestion.
Study objective
The objective of this study is to investigate the feasibility of using MT and
CEST MRI for in vivo monitoring of gastric protein digestion (coagulation and
breakdown).
This will be done by studying the effect of heating on gastric protein
coagulation and breakdown using pasteurized and extensively heated milk as a
test case.
Study design
Randomized cross-over study with two treatments (pasteurized and extensively
heated skim milk). Participants will visit the hospital Gelderse Vallei two
times in a fasted state. First, a baseline MRI scan will be made, following
this, they have to drink 300 mL of milk and MRI scans will be made up until 95
min. In the two visit the participants will have to drink to two different
types of milk products.
Study burden and risks
The risks associated with participation are low because MRI is a safe
technique, and the test foods consist of commercial milk products. The burden
associated with participation consists of two visits, which include an
overnight fast and MRI scans for 95 mins. These may all cause minimal
discomfort. There is no benefit of participation for the participants. The
study will be performed with healthy participants.
Stippeneng 4
Wageningen 6708WE
NL
Stippeneng 4
Wageningen 6708WE
NL
Listed location countries
Age
Inclusion criteria
18-45 years old
Apparently healthy
Normal weight (BMI 18.5-25 kg/m2)
Exclusion criteria
• Milk protein allergy or intolerance y (self-reported) • Lactose intolerance
(self-reported) • Gastric disorders or regular (>once a week) gastric
complaints such as heartburn • Use of medication which alters the normal
functioning of the stomach, such as: - medical drug use that influences the GI
tract*s normal function, e.g. the motility, pH etc: among others use of proton
pump inhibitors, antacids, anti-depressants etc. • Being pregnant, lactating or
planning on becoming pregnant during the study • Use of recreational drugs
within one week prior to the test day (marihuana, XTC, GHB, helium) • Alcohol
consumption of more than 7 glasses/per week • Smoking (>2 cigarettes a week)
• Having gained or lost more than 5 kg weight in the last month. •
Participating in other biomedical research during the study period • Having a
contra-indication to MRI scanning (including, but not limited to): -
Intraorbital or intraocular metallic fragments - Ferromagnetic implants -
Claustrofobia (self-reported) • Unwillingness to be referred to my general
practitioner in case of a chance finding of pathology • Being an employee or
thesis student of the Division of Human Nutrition and Health or the Laboratory
of Biophysics at Wageningen University.
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL83133.091.23 |
| Other | tbd |