We aim to investigate how chromosomal mosaicism affects development of the different embryonic and extra-embryonic tissues of the peri-implantation blastocyst and early post-implantation embryo to understand why some mosaic embryos can continue…
ID
Source
Brief title
Condition
- Foetal complications
- Sexual function and fertility disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Outcome parameters include embryo morphology before and after culture, live
staining for developmental markers, molecular karyotyping, RNA or protein
expression of markers for lineage establishment and signalling pathway
activation.
Secondary outcome
To establish the molecular interactions between trophoblast, hypoblast and
epiblast lineages that regulate their developmental progression, we will
combine findings from live imaging analysis, immunofluorescence analysis,
single cell transcriptomics analysis and single-cell epigenetic profiles to
identify timing and activity of pathways involved.
To test these pathways and to explore candidate extraneous signals that can
support pre-implantation development, functional assessment will be performed
using the main study parameters as read outs.
Background summary
Despite significant improvements in culture conditions and selection of embryos
from in vitro fertilization (IVF) treatment, success rates have only marginally
improved, and the rate of pregnancy loss shortly after implantation remains
high. We were among the first to report on the high incidence of chromosomal
abnormalities in human cleavage stage IVF embryos. The majority of these
embryos display chromosomal mosaicism, a mixture of cells with normal and
abnormal chromosomal constitutions. Although the incidence of mosaicism
declines towards the blastocyst stage, aneuploid cells can persist at this
stage where it is associated with a decreased implantation potential. However,
some mosaic embryos can overcome the presence of aneuploid cells and result in
healthy live births.
Study objective
We aim to investigate how chromosomal mosaicism affects development of the
different embryonic and extra-embryonic tissues of the peri-implantation
blastocyst and early post-implantation embryo to understand why some mosaic
embryos can continue development and others do not.
Study design
This is an observational study using molecular and imaging techniques to
investigate surplus human embryos after in vitro fertilization treatment.
Study burden and risks
The study will not interfere with the standard IVF- and embryo transfer
procedures and will only use surplus embryos. The study will not negatively
affect pregnancy rates, nor will it affect the women*s or children*s health.
The outcome of these studies will increase understanding of the incidence and
consequences of chromosomal abnormalities, and contribute knowledge to improve
culture and selection of healthy embryos for future IVF patients.
Dr. Molewaterplein 40
Rotterdam 3015 GD
NL
Dr. Molewaterplein 40
Rotterdam 3015 GD
NL
Listed location countries
Inclusion criteria
Eligible embryo donors to donate embryos for this study meet the following
criteria:
- The availability of surplus (poor quality) fresh or cryopreserved embryos;
- Signed informed consent form to donate surplus embryos for research
- Age >= 18 years
Exclusion criteria
A potential subject who meets any of the following criteria will be excluded
from participation in this study:
- Informed consent form only signed by one of the gamete providers or
intentional parents
- No surplus embryos
- Surplus embryos with excessive degeneration or fragmentation (>50%)
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL82597.000.22 |