To assess protein-specific synthesis rates neocortex, hippocampus, temporalis muscle, and vastus lateralis muscle using the D2O dosing method.
ID
Source
Brief title
Condition
- Musculoskeletal and connective tissue disorders NEC
- Neurological disorders NEC
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
Primary study parameters include protein synthesis rates and enrichments of
neocortex, hippocampus, temporalis muscle, and vastus lateralis muscle.
Secondary outcome
Secondary parameters include whole-body protein synthesis, breakdown,
oxidation, and net balance.
Background summary
Brain plasticity is defined by a dynamic balance between protein synthesis and
protein breakdown rates. Recently, human brain tissue from a pilot project has
shown to turn over at a rate of ~3% per day using contemporary stable isotope
methodology. However, assessment of these turnover rates represents the
integrated synthesis rates of all available protein in brain tissue and does
not allow assessment of synthesis rates on the level of individual proteins.
Therefore, this project will apply the D2O dosing method primarily to assess
protein turnover rates of different parts of the human brain. In addition,
using D2O methodology will enable us to assess protein turnover on the level of
individual proteins.
Study objective
To assess protein-specific synthesis rates neocortex, hippocampus, temporalis
muscle, and vastus lateralis muscle using the D2O dosing method.
Study design
Explorative study design.
Study burden and risks
The risks involved in participating in this study are minimal. We will only
make use of tissue that is being resected as part of the surgical procedure.
Saliva sampling is risk-free and blood sampling is minimal (8-12 x 10mL total)
and will be conducted using catheter placement during the dosing day and the
day of the surgical procedure (1 blood sample and 4-8 samples, respectively).
The vastus lateralis biopsy will be obtained by an experienced physician during
the surgical procedure using the Bergström percutaneous needle biopsy method.
Universiteitssingel 50
Maastricht 6229ER
NL
Universiteitssingel 50
Maastricht 6229ER
NL
Listed location countries
Age
Inclusion criteria
* Written informed consent
* Male and female patients scheduled for temporal lobectomy
* Age 18 - 65 years
* Compos mentis
Exclusion criteria
* Comorbidities and neuromuscular disorders of the lower limbs severely
interacting with mobility, with limited or no opportunity for improvement (e.g.
cerebral palsy)
* Peripheral artery disease Fontaine III or IV
* Chronic Obstructive Pulmonary Disease (COPD) GOLD III or IV
* Use of systemic steroids in the past four weeks, other than indicated for the
specific type of surgery
* Use of anti-inflammatory biologicals (e.g. TNF-alfa blockers) in the past
four weeks
* Surgical intervention in the past four weeks
* Total parenteral nutrition at day of surgery
* Pregnancy
* Neoadjuvant chemotherapy or radiotherapy
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL63300.068.17 |