To determine the amino acid metabolic availability of protein in milk, sorghum and black beans in older (65-80 years) and younger (20-35 years) men using the IAAO method
ID
Source
Brief title
Condition
- Other condition
Synonym
Health condition
veroudering
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
13C-enrichment in breath samples will be expressed in APE. CO2 production rate
(VCO2) will be measured by indirect calorimetry and expressed in mL/min. The
rate of 13CO2 production (F13CO2) will be calculated using these two
measurements. The slope for the graded intakes of the limiting amino acid and
the oxidation level will be constructed for the reference and the test
proteins. The ratio between the reference and test protein will be calculated
to represent metabolic availability of the limiting amino acid in each protein
source.
Secondary outcome
Not applicable
Background summary
Meeting amino acid requirements is one of the factors that is important for
older adults to prevent development of sarcopenia. Dietary protein quality is
of importance, as determined by amino acid composition and bioavailability of
the amino acids from the consumed protein. The effect of ageing on metabolic
availability of indispensable amino acids of foods in humans is not well
studied. The indicator amino acid oxidation (IAAO) method is an indirect,
non-invasive method to determine amino acid metabolic availability in humans.
Study objective
To determine the amino acid metabolic availability of protein in milk, sorghum
and black beans in older (65-80 years) and younger (20-35 years) men using the
IAAO method
Study design
A postprandial dietary intervention study will be performed using the IAAO
method. Participants will come either 7 times to test metabolic availability of
methionine in beans or 10 times to test metabolic availability of lysine in
milk and sorghum.
Intervention
Graded intakes of the limiting amino acid (lysine or methionine) in a reference
amino acid mixture or the test protein source (milk, sorghum or black beans)
will be consumed by the participants on different test days. One test day
equals one intake level of the limiting amino acid which meal is divided over 9
hourly portions. 13C-labelled phenylalanine (Phe) is supplied from the 5th hour
onwards. Breath samples will be taken regularly.
Study burden and risks
Research in older adults is necessary to provide them with suitable nutritional
advice. The participants will not benefit directly from participating in this
study. Stable isotopes occur in nature, are not radioactive and are not harmful
for the subjects. The burden of this study concerns the obliged consumption of
meals and time investment of the test days.
Stippeneng 4
Wageningen 6708WE
NL
Stippeneng 4
Wageningen 6708WE
NL
Listed location countries
Age
Inclusion criteria
- Gender: male
- Age: 20-35 years or 65-80 years
- Body Mass Index (BMI): 18.5 - 30.0 kg/m2
- Healthy as assessed with a questionnaire
- Regular and normal eating habits as assessed with a Food Frequency
Questionnaire (FFQ)
- Willing to comply with study procedures and use of data
- Having given written informed consent
Exclusion criteria
Chronic disease, for example: - Diabetes mellitus / being treated for high
blood glucose - Severe cardiovascular disease (exception: hypertension) -
Hepatic disease (e.g. hepatitis) - Renal disease - Cancer - Bowel disease (e.g.
inflammatory bowel disease, ulcers, bleeding) - Pancreatitis
History of medical or surgical events that may affect GI function, study
outcomes or disease risk for participants, for example: - Bariatric surgery -
Gastrointestinal tract surgery - Digestive tract disorder - Chewing problems -
History of deep venous thrombosis without anti-coagulation medication
Medicine use that interferes with, protein metabolism, GI function and the
study outcomes, for example: - Glucose lowering drugs - Proton pump inhibitors
- Laxatives
Habits that interfere with the study outcomes: - Protein supplement use
(current use or less than 2 weeks past use) - Smoking - Drug abuse - Alcohol
consumption >21 units/week or >4/day - Following a weight-loss diet, medically
prescribed diet or other diet with a low calorie intake or an unbalanced
nutrient intake like a vegan or very low carbohydrate diet - Moderate to high
intense physical activity for more than 5 hours a week
Other: - Self-reported allergy or intolerance to the tested products - Weight
loss of more than 3 kg in the 3 months prior to study screening - Current
participation in other research and <2 months prior participation in other
research - Not having a general practitioner - Not willing to accept
information-transfer concerning participation in the study, or information
regarding his or her health to his general practitioner - Working or doing a
MSc thesis at the department of Human Nutrition and Health at Wageningen
University and Research
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL84684.091.23 |