To characterize the molecular mechanisms underlying parasympathetic regulation of adipose tissue.
ID
Source
Brief title
Condition
- Glucose metabolism disorders (incl diabetes mellitus)
- Lipid metabolism disorders
Synonym
Research involving
Sponsors and support
Intervention
Outcome measures
Primary outcome
The adipose tissue specimens will be used for the determination of the
following parameters: mRNA measurements by real time PCR, protein
identification by western blot and immunohistochemistry, glycerol release
(lipolysis) and cytokine release from isolated adipocytes, adipocyte size, cell
type identification in adipose tissue by FACS analysis, signal transduction
analysis in isolated adipocytes using specific agonists and antagonists in
vitro.
Secondary outcome
not applicable
Background summary
Obesity is a serious health problem with epidemic proportions. Fatty acids and
adipokines that are released by the expanding adipose tissue mass play a key
role in the development of type 2 diabetes and cardiovascular diseases.
However, the molecular mechanisms responsible for adipose tissue (dys)function
have yet to be fully characterized.
This study will investigate the molecular mechanisms underlying parasympathetic
regulation of adipose tissue.
Study objective
To characterize the molecular mechanisms underlying parasympathetic regulation
of adipose tissue.
Study design
In human subcutaneous and visceral adipose tissue and adipocytes
1) receptors of parasympathetic neurotansmitters or neuropeptides will be
identified,
2) signaling pathways by which the neurotransmitters exert their effects will
be investigated in vitro,
3) effects of these signaling pathways on metabolic and endocrine function will
be studied in vitro.
Study burden and risks
There is no burden for the patient other than the surgery for their disorder.
Within 30 minutes after the abdominal wall is opened for surgery a specimen of
~5-g of subcutaneous and visceral adipose tissue will be obtained. There is no
risk associated with the removal of the tissue.
S-04-P, Postbus 9600
2300 RC Leiden
NL
S-04-P, Postbus 9600
2300 RC Leiden
NL
Listed location countries
Age
Inclusion criteria
Patients referred for open elective abdominal surgery for hernia or gall stones which are healthy except for surgical diagnosis
Exclusion criteria
Any significant infectious disease
Any significant chronic disease
Minors aged under18 y
Incapacitated adults
Design
Recruitment
Followed up by the following (possibly more current) registration
No registrations found.
Other (possibly less up-to-date) registrations in this register
No registrations found.
In other registers
| Register | ID |
|---|---|
| CCMO | NL29149.058.09 |