The effect of dopamine depletion on endogenous glucose production in healthy subjects.

It has been hypothesized that dopamine may be involved in the modulation of glucose metabolism. This hypothesis is partly based on the observation of the relationship between schizophrenia and diabetes and the finding that lean drug-naive schizophrenic patients display hepatic insulin resistance.

We will investigate glucose metabolism in healthy subjects after dopamine depletion with AMPT and placebo, using a hyperinsulinemic euglycemic clamp with stable isotopes.

Hepatic insulin sensitivity increase after dopamine depletion, with a-methylparatyrosine (AMPT).

We will draw bloodsamples in the basal state, after de first and after the second step of the clamp.

Each subject will be studied twice. Once after dopamine depletion (with AMPT) and once after placebo. We will perform a 2-step hyperinsulinemic euglycemic clamp, using stable isotopes.