We hypothesize that genetically programmed developmental differences in adipocytes are under direct regulation of fecal flora composition which thus plays an important role in obesity, body fat distribution ans systhemic inflammation.
ID
Bron
Verkorte titel
Aandoening
gut microbiota, obesity, fat adipose tissue
Ondersteuning
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
Expression of genes in adipose tissue will be compared to gut microbiota composition.
Achtergrond van het onderzoek
Objective: To investigate correlations between gene expression in adipocytes and gut microbiota composition in relation to plasma markers of inflammation.
Study design: Cross-sectional design.
Study Population: Healthy men and women scheduled for elective laparoscopic cholecystectomy.
Outcome measures: Expression of human genes will be first tested on Affimetrix microarrays and genes of interest will thereafter be assessed by quantitative real-time RT-PCR. These genes in adipose tissue will be compared to gut microbiota composition. Finally we will try to relate regulation of genes to plasma markers of inflammation.
Sample Size: It is estimated that a total of 200 patients (100 males and 100 females) are needed.
Doel van het onderzoek
We hypothesize that genetically programmed developmental differences in adipocytes are under direct regulation of fecal flora composition which thus plays an important role in obesity, body fat distribution ans systhemic inflammation.
Onderzoeksopzet
Cross-sectional design, only 1 timepoint.
Onderzoeksproduct en/of interventie
During the planned surgery visceral and subcutaneous biopsies will be obtained. In all patients venous blood samples will be taken and the patients will collect a feces sample.
Algemeen / deelnemers
A. Vrieze
Academic Medical Center, room F4-256
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5665983
a.vrieze@amc.uva.nl
Wetenschappers
A. Vrieze
Academic Medical Center, room F4-256
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5665983
a.vrieze@amc.uva.nl
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
1. Healthy men and women, scheduled for elective laparoscopic cholecystectomy;
2. Age 18-85 yrs;
3. BMI 18-45 kg/m2.
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
1. Generalized inflammation;
2. Antibiotic use last three months;
3. Malignant disease;
4. Metformin use.
Opzet
Deelname
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