Fatty Acid Induced Oxidative Stress: it's role in preventing hypoglycemia.

During fasting hypoglycemia must be prevented to protect glucosedependent tissues. Insulin resistance is a mechanism to do so. Free fatty acids play a major role in this process. Also there is a role for oxidative stress that inhibits insulin mediated glucose uptake by several proposed mechanisms. Lipolysis can be inhibited bij acipimox wich will result in a decrease in FFA levels. Hypothetically FFA inhibition decreases oxidative stress with a concomitant decrease in insulin resistance.

Elevated levels of Free Fatty Acids during fasting induce oxidative stress and cause insulin resistance to maintain euglycemia.

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Subjects will undergo a period of fasting and are assigned to receive either acipimox (inhibitor lipilysis) 250mg 4dd or placebo. Hereafter insulin sensitivity will be measured using stable isotope technique. Furthermore regulating hormones and lipids will be measured. Muscle specimens (v. lateralis) will be obtained for determination of intramyocellular lipids and transscription factors.