Does cycling exercise induce increased coagulation activity through contact activation?
ID
Bron
Aandoening
Exercise, coagulation, platelet reactivity, thrombin generation, endofibrosis, cycling
Ondersteuning
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
Expecting to show increased coagulation activity by increased TAT levels, supported by decreased Coagulation Time (CT) and enhanced Maximal clotting formation (MCF) by Rotem and enhanced thrombin generation through CAT assay. Increased fibrinolytic activity is expected by increased tPA and D-Dimer levels. Coagulation activation via the intrinsic pathway can be showed by enhanced thrombin generation in Active Side Inhibitor factor Seven (ASIS) assay. Increased platelet reactivity is expected by multiplate accompanied by a increase in platelet count. Moreover we expect increase vWf levels, due to endothelial dysfunction.
Achtergrond van het onderzoek
Although a clear association between exercise and activation of coagulation has been demonstrated, evidence is fragmented and the trigger remains unknown. Given the protease activated receptor (PAR) activation by coagulation proteases and the subsequent cellular effects such as inflammation, migration and apoptosis, haemostatic changes during exercise may contribute to the development of endofibrosis in cyclists. This pathology is characterized by intimal thickening of the iliacal artery, with reduced blood flow as a consequence.
Doel van het onderzoek
Does cycling exercise induce increased coagulation activity through contact activation?
Onderzoeksopzet
March 2013: Start of study.
Every 2 weeks a group of 5 cyclists and controle groupe will be tested. So 20 cyclists and 20 controle group members will be tested within 2 months.
Onderzoeksproduct en/of interventie
Blood will be collected before and after long-term strenuous exercise. Coagulation profile will be measured bij Thrombin Generation (CAT), Rotem;Natem and by measuring Thrombin-Antithrombin complex , Coagulation Factor XIa (FXIa), tissue Plasminogen Activator (tPA), D-Dimer and von Willebrand Factor (vWf). Platelet reactivity will be assesed by Multiplate. Blood cell counts are determined for testing the full blood cell count profile by: Hematocrit, Haemoglobin, platelets, cortisol and leukocytes (differentiation).
Publiek
Room 4.330
Jelle Posthuma
Maastricht 6800 MD
The Netherlands
+31 (0)43 3881542
jelle.posthuma@student.maastrichtuniversity.nl
Wetenschappelijk
Room 4.330
Jelle Posthuma
Maastricht 6800 MD
The Netherlands
+31 (0)43 3881542
jelle.posthuma@student.maastrichtuniversity.nl
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
1. Minimal 18 years old;
2. Trains more than 3 times a week;
3. Mentally competent.
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
1. Diagnosed with coagulation or platelet disorder;
2. Using medication against coagulation or platelet function;
3. Vascular operation within 6 months before the research;
4. BMI > 30;
5. Prestation influencing drugs;
6. Pregnancy.
Opzet
Deelname
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In overige registers
| Register | ID |
|---|---|
| NTR-new | NL3676 |
| NTR-old | NTR3846 |
| CCMO | NL42855.068.12 |
| ISRCTN | ISRCTN wordt niet meer aangevraagd. |
| OMON | NL-OMON38959 |