Evaluation of a dentifrice with natural ingredients in the prevention of plaque and gingivitis

The study is designed to evaluate the effect of a dentifrice with natural ingredients over a period of 4 months. 90 subjects (non-dental) will be selected on the basis of having moderate gingival inflammation. The single blind study consists of 2 phases: a pre-experimental phase of 3 weeks and an experimental period of 4 months. At the start of the pre-experimental phase gingivitis (MGI) bleeding upon marginal probing (BOMP) and plaque (Quigley & Hein) will be assessed. Subjects receive a written instruction in the use of a manual toothbrush. Furthermore, a combination of Bocasan® and chlorhexidine 0.20% will be used to rinse 2 times per day during the 3 weeks prior to the second fase of the experiment. The baseline assessment is scheduled 3 weeks later. Subjects are randomly assigned to one of 2 groups (test & control). All will be instructed to brush their teeth with their assigned toothpaste for 2 minutes 2 times a day using the timer. Clinical examinations will be performed after 4 months. All parameters assessed at the start of the study are evaluated after 4 months.

The dentifrice with natural ingredients (Verum) inhibit 10% less gingivitis compared to the placebo dentifrice over a period of 4 months in healthy subjects.

-Pre-experimental phase (3 weeks)

-Experimental phase

-baseline

-4 months

Pre-experimental phase (3 weeks):

Brushing 2x a day:-Everclean toothpaste (a standard toothpaste)and -Aronal oko-dent toothbrush.

Rinsing 2x a day: - 0,2% Chloorhexidine mouthrinse (Corsodyl®) and 10 ml waterperoxide-oplossing (Bocasan®)

Experimental phase (4 months):

Brushing 2x a day:-Verum toothpaste(TEST OR CONTROL) in combination with the Aronal oko-dent toothbrush