Effects of low-fat vs high-fat diet on lipid accumulation in liver and skeletal muscle in overweight men.

1. Male sex;

2. Age 40-65 years;

3. BMI 25-35 kg/m2;

4. Sedentary;

5. Stable dietary habits;

6. Healthy.

1. Current use of medication that is known to interfere with the results of the study;

2. Consuming more than 20 g of alcohol per day (± 2 units);

3. Serum-γ-glutamyltranspeptidase level > 70 IU/L;

4. A history of cardiovascular disease like congestive heart failure or acute myocardial infarction;

5. Plasma triacylglycerol > 4.5 mmol/L;

6. Familial hypercholesterolemia;

7. A history of liver disease;

8. Unstable body weight (weight gain or loss > 3 kg in the past three months);

9. Abuse of drugs;

10. Participation in another biomedical study within 1 month prior to the screening visit;

11. Impossible or difficult venipuncture during screening;

12. A fasting glucose above 7.0 mmol/L (13);

13. A contraindication to MRI scanning. These contraindications include patients with the following devices:

a. Central nervous system aneurysm clips;

b. Implanted neural stimulator;

c. Implanted cardiac pacemaker or defibrillator;

d. Cochlear implant;

e. Ocular foreign body (e.g. metal shavings);

f. Insulin pump;

g. Metal shrapnel or bullet;

h. Or metal containing corpora aliena in the eye of brains.