We hypothesized that FFA loading could impair mitochondrial function and that the potency of FFA to disturb mitochondrial function and glucose uptake would depend on the degree of desaturation of the fatty acids infused.
ID
Bron
Verkorte titel
Aandoening
Diabetes, Insulin resitance and mitochondrial dysfunction
Ondersteuning
Onderzoeksproduct en/of interventie
Uitkomstmaten
Primaire uitkomstmaten
- Peripheral glucose uptake (Rd) <br>
- Resting, adp-stimulated and uncoupled mitochondrial respiration rates
Achtergrond van het onderzoek
Recently, disturbed mitochondrial function was shown to be associated with free fatty acid (FFA)- and obesity induced insulin resistance. Different types of FFA can have differential effects on glucose metabolism. Therefore we evaluate the effects of elevated plasma monounsaturated vs. polyunsaturated fatty acids on glucose metabolism and mitochondrial function.
Doel van het onderzoek
We hypothesized that FFA loading could impair mitochondrial function and that the potency of FFA to disturb mitochondrial function and glucose uptake would depend on the degree of desaturation of the fatty acids infused.
Onderzoeksopzet
Participants were studied in balanced assignment on three different occasions (each two weeks apart) during a 6 hours infusion of Intralipid ®, Clinoleic®, or saline 0.9% intravenously
Onderzoeksproduct en/of interventie
Infusion with with to different lipid emulsion respectivily Intralipid® and Clinoleic®, versus Saline. Each patient is his own control.
Publiek
F5-162 Endocrinology and Metabolism
M. Brands
Meibergdreef 9
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5662663
m.brands@amc.uva.nl
Wetenschappelijk
F5-162 Endocrinology and Metabolism
M. Brands
Meibergdreef 9
Amsterdam 1100 DD
The Netherlands
+31 (0)20 5662663
m.brands@amc.uva.nl
Belangrijkste voorwaarden om deel te mogen nemen (Inclusiecriteria)
1. Healthy male volunteer
2. Caucasian
3. Age between 18-35 years
4. Normal BMI ( 20-24.9 kg/m2)
5. VO2 max (90-110% predicted value according to Jones formula)
6. Stable weight during 3 months before participation and during participation
7. Normal physical activity with at least 2 times per week sport activities
8. Non-smoking
Belangrijkste redenen om niet deel te kunnen nemen (Exclusiecriteria)
1. Fasting blood glucose > 5,6 mmol/L or abnormal OGTT (2-hour value after oral ingestion of 75 g, glucose >7.8 mmol/l) Fasting insulin concentration >50 pmol/l
2. BMI> 25 and BMI< 20
3. DM type II in first degree relatives
4. All chronic diseases
5. Drug use
6. Dyslipidaemia
7. Abnormal liver or renal function
Opzet
Deelname
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| Register | ID |
|---|---|
| NTR-new | NL1439 |
| NTR-old | NTR1500 |
| Ander register | : MEC 05/295 |
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